Transcriptomic Profile Analysis of Response to Flower Extracts.

BACKGROUND

has been used as a traditional medicine to treat oral health diseases such as dental caries and periodontitis. is a common bacterium that initiates dental caries at an early stage.

AIM

The aim of this study was to determine the mode of action of . (extracts) against . growth.

METHODS

Time-kill assay has been done to investigate the rate of kill and effectiveness of (AP) extracts against . growth. Phytochemical analysis was done to identify major compounds in AP extracts using gas chromatography mass spectrometry (GCMS). Scanning and transmission electron microscopy (SEM and TEM) have been done to observe the morphological changes of treated bacteria. Transcriptomic profile analysis has been done using Next Gene Sequencing.

RESULTS

AP flower n-hexane (APFH) and AP flower dichloromethane (APFD) extracts acted as bactericidal agents after killing >3 log10 cfu/mL of after 24 hours. Oleic and hexadecenoic acids were found to be the major compounds in APFD and APFH extracts, respectively. Photomicrographs from SEM and TEM of treated . show that the bacterial cell wall has been lysed and the cytoplasm content was decreased. Pathway analysis revealed that the APFD extract significantly affected biosynthesis peptidoglycan, gene expression, RNA processing, and macromolecule metabolism processes in . .

CONCLUSION

Data analysis revealed that multiple mechanisms of action were involved in antibacterial activity of . extracts toward . .