Stanek G, Burger I, Hirschl A, Wewalka G, Radda A
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):29-33. doi: 10.1016/s0176-6724(86)80098-0.
Ticks of the species Ixodes ricinus were cultured in the laboratory. Yellow silver rabbits, gerbils and white mice served as blood hosts. Borrelia burgdorferi could be detected by means of an IFA test in homogenates of female ticks, their eggs as well as the respective larval and nymphal ticks. Blood infection of splenectomized gerbils and ordinary white mice or of ordinary white mice alone has been demonstrated after feeding of larval or nymphal ticks on them, respectively. Spirochetemia started 5 to 8 days after feeding and lasted for ca 3 weeks. Two distinct peaks in the cell count of spirochetal organisms per ml blood plasma could be observed on days 11-13 (5 X 10(5) to 2 X 10(6) cells/ml) and 17-19 (10(5) cells/ml), regardless whether splenectomized gerbils or white mice were used. The results display that B. burgdorferi is vertically from the female ticks to their eggs and transstadially transmitted. The transmission-rate from larval to nymphal ticks is 100%. These findings show the tick itself as a main reservoir of B. burgdorferi. The established mouse-model appears to be a useful tool to detect Borrelia carrying ticks.
在实验室中培养蓖麻硬蜱。黄银兔、沙鼠和小白鼠作为血源宿主。通过间接荧光抗体试验（IFA），可在雌性蜱、其卵以及相应的幼虫和若虫蜱的匀浆中检测到伯氏疏螺旋体。分别用幼虫或若虫蜱叮咬脾切除的沙鼠和普通小白鼠或仅普通小白鼠后，已证实它们发生了血液感染。螺旋体血症在进食后5至8天开始，持续约3周。无论使用脾切除的沙鼠还是小白鼠，在第11 - 13天（5×10⁵至2×10⁶个细胞/毫升）和第17 - 19天（10⁵个细胞/毫升），每毫升血浆中螺旋体生物的细胞计数可观察到两个明显的峰值。结果显示，伯氏疏螺旋体可从雌性蜱垂直传播至其卵，并经变态期传播。从幼虫到若虫蜱的传播率为100%。这些发现表明蜱本身是伯氏疏螺旋体的主要储存宿主。所建立的小鼠模型似乎是检测携带伯氏疏螺旋体蜱的有用工具。
