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Integrating thermodynamics and molecular kinetics into western blotting teaching.

作者信息

Wang Zhiyong, Wang Min, Yu Yan, Lu Yanxin, Xia Qiang, Wei Pei

机构信息

Department of Immunology, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.

Department of Pharmaceutics, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.

出版信息

Front Mol Biosci. 2025 Aug 14;12:1640740. doi: 10.3389/fmolb.2025.1640740. eCollection 2025.

DOI:10.3389/fmolb.2025.1640740
PMID:40894074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12390805/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b822/12390805/407a5ae7f966/fmolb-12-1640740-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b822/12390805/b22908e15c57/fmolb-12-1640740-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b822/12390805/407a5ae7f966/fmolb-12-1640740-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b822/12390805/b22908e15c57/fmolb-12-1640740-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b822/12390805/407a5ae7f966/fmolb-12-1640740-g002.jpg

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本文引用的文献

1
Comprehensive Optimization of Western Blotting.蛋白质印迹法的全面优化
Gels. 2023 Aug 14;9(8):652. doi: 10.3390/gels9080652.
2
B cell receptors and free antibodies have different antigen-binding kinetics.B 细胞受体和游离抗体具有不同的抗原结合动力学。
Proc Natl Acad Sci U S A. 2023 Aug 29;120(35):e2220669120. doi: 10.1073/pnas.2220669120. Epub 2023 Aug 24.
3
Western Blotting with Fast SDS-PAGE and Semi-Dry Protein Transfer.快速 SDS-PAGE 和半干蛋白质转移的 Western Blotting。
Curr Protoc. 2023 Jul;3(7):e833. doi: 10.1002/cpz1.833.
4
Western blotting (immunoblotting): history, theory, uses, protocol and problems.Western 印迹法(免疫印迹法):历史、理论、用途、方案和问题。
Biotechniques. 2023 Sep;75(3):99-114. doi: 10.2144/btn-2022-0034. Epub 2023 Mar 27.
5
Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them.免疫印迹中的盲区:对免疫印迹图谱和方法报告中常见问题的评估,并提出改进建议。
PLoS Biol. 2022 Sep 12;20(9):e3001783. doi: 10.1371/journal.pbio.3001783. eCollection 2022 Sep.
6
Western blotting: a powerful staple in scientific and biomedical research.蛋白质免疫印迹法:科学和生物医学研究中一项强大的常用技术。
Biotechniques. 2022 Jun;73(1):58-69. doi: 10.2144/btn-2022-0003. Epub 2022 Jul 1.
7
Western Blot Processing Optimization: The Perfect Blot.Western Blot 处理优化:完美印迹。
Methods Mol Biol. 2022;2349:65-80. doi: 10.1007/978-1-0716-1585-0_4.
8
The SARS CoV-2 spike directed non-neutralizing polyclonal antibodies cross-react with Human immunodeficiency virus (HIV-1) gp41.SARS-CoV-2 刺突蛋白诱导的非中和性多克隆抗体与人类免疫缺陷病毒 (HIV-1) gp41 发生交叉反应。
Int Immunopharmacol. 2021 Dec;101(Pt B):108187. doi: 10.1016/j.intimp.2021.108187. Epub 2021 Sep 28.
9
The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding.抗 DNA 抗体与 DNA 抗原的相互作用:高亲和力结合的滞后证据。
Clin Immunol. 2021 Oct;231:108848. doi: 10.1016/j.clim.2021.108848. Epub 2021 Sep 4.
10
Evaluation of Binding Kinetics and Thermodynamics of Antibody-Antigen Interactions and Interactions Involving Complement Proteins.评估抗体-抗原相互作用和涉及补体蛋白的相互作用的结合动力学和热力学。
Methods Mol Biol. 2021;2227:237-247. doi: 10.1007/978-1-0716-1016-9_21.