硫酸葡聚糖作为从浓缩病毒中提取的DNA的污染物以及DNA聚合酶的抑制剂。
Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.
作者信息
Hitzeman R A, Hanel A M, Price A R
出版信息
J Virol. 1978 Jul;27(1):255-7. doi: 10.1128/JVI.27.1.255-257.1978.
Abstract
Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.
摘要
硫酸葡聚糖通常与聚乙二醇一起用于在提取病毒DNA之前浓缩病毒。然而，硫酸葡聚糖随后很容易污染此类DNA，并作为枯草芽孢杆菌（III）、噬菌体PBS2和噬菌体T4的DNA聚合酶的强效抑制剂。硫酸葡聚糖仅对藤黄微球菌和大肠杆菌DNA聚合酶I制剂有微弱抑制作用。
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