羟基苯基偶氮嘧啶对枯草芽孢杆菌DNA聚合酶的抑制作用。
Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines.
作者信息
Gass K B, Low R L, Cozzarelli N R
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):103-7. doi: 10.1073/pnas.70.1.103.
Abstract
The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a K(i) of less than 1 muM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.
摘要
抗菌剂6 - （对羟基苯基偶氮）尿嘧啶能特异性抑制枯草芽孢杆菌的DNA聚合酶III，其抑制常数（K(i)）小于1微摩尔。这种抑制作用需要药物预先还原，是可逆的，并且与dGTP具有竞争性。高浓度的dATP可防止密切相关的药物6 - （对羟基苯基偶氮）异胞嘧啶产生抑制作用。本文提出了一个模型，其中抑制剂在与酶形成三元复合物的同时与模板进行碱基配对。这些结果表明，枯草芽孢杆菌的DNA聚合酶III对染色体复制是必需的。
相似文献
1
Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines.羟基苯基偶氮嘧啶对枯草芽孢杆菌DNA聚合酶的抑制作用。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):103-7. doi: 10.1073/pnas.70.1.103.
2
Inhibition of a discrete bacterial DNA polymerase by 6-(p-hydroxyphenylazo)-uracil and 6-(p-hydroxyphenylazo-)-isocytosine.6-（对羟基苯偶氮基）尿嘧啶和6-（对羟基苯偶氮基）异胞嘧啶对一种特定细菌DNA聚合酶的抑制作用
Nat New Biol. 1972 Nov 15;240(98):80-2. doi: 10.1038/newbio240080a0.
3
Hydroxyphenylazopyrimidines: characterization of the active forms and their inhibitory action on a DNA polymerase from Bacillus subtilis.羟基苯基偶氮嘧啶：活性形式的表征及其对枯草芽孢杆菌DNA聚合酶的抑制作用
Proc Natl Acad Sci U S A. 1973 Feb;70(2):512-6. doi: 10.1073/pnas.70.2.512.
4
5
Effect of 6-(p-hydroxyphenyl)-azouracil on B. subtilis DNA polymerases.6-（对羟基苯基）-偶氮尿嘧啶对枯草芽孢杆菌DNA聚合酶的影响。
Nat New Biol. 1972 Nov 15;240(98):82-3. doi: 10.1038/newbio240082a0.
6
Mutational alteration of Bacillus subtilis DNA polymerase 3 to hydroxyphenylazopyrimidine resistance: polymerase 3 is necessary for DNA replication.枯草芽孢杆菌DNA聚合酶3对羟基苯偶氮嘧啶抗性的突变改变：DNA复制需要聚合酶3 。
Biochem Biophys Res Commun. 1973 Mar 5;51(1):151-7. doi: 10.1016/0006-291x(73)90521-4.
7
Inhibition of bacterial DNA replication by 6-(p-hydroxy-phenylazo)-uracil.6-（对羟基苯偶氮）尿嘧啶对细菌DNA复制的抑制作用
Nat New Biol. 1972 May 17;237(72):72-4. doi: 10.1038/newbio237072a0.
8
Mechanism of inhibition of Bacillus subtilis DNA polymerase 3 by the arylhydrazinopyrimidine antimicrobial agents.芳基肼嘧啶类抗菌剂对枯草芽孢杆菌DNA聚合酶3的抑制机制。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2973-7. doi: 10.1073/pnas.71.8.2973.
9
A comparison of the utilization of thymine and thymidine as precursors for deoxyribonucleic acid synthesis in Bacillus subtilis, and their specific inhibition by 6-(p-hydroxyphenylazo)uracil and caffeine.枯草芽孢杆菌中胸腺嘧啶和胸腺嘧啶核苷作为脱氧核糖核酸合成前体的利用情况比较，以及它们被6-（对羟基苯偶氮）尿嘧啶和咖啡因特异性抑制的情况。
Biochem J. 1972 Sep;129(3):49P-50P. doi: 10.1042/bj1290049pb.
10
Temperature dependent permeability changes of Bacillus subtilis and incorporation of nucleotides into DNA.枯草芽孢杆菌的温度依赖性通透性变化及核苷酸掺入DNA的过程
Biochem Biophys Res Commun. 1973 Dec 10;55(3):788-97. doi: 10.1016/0006-291x(73)91213-8.
引用本文的文献
1
RecO and RecR are necessary for RecA loading in response to DNA damage and replication fork stress.RecO 和 RecR 对于 RecA 在应对 DNA 损伤和复制叉压力时的加载是必要的。
J Bacteriol. 2014 Aug;196(15):2851-60. doi: 10.1128/JB.01494-14. Epub 2014 Jun 2.
2
Nucleoid occlusion prevents cell division during replication fork arrest in Bacillus subtilis.核小体阻遏物在枯草芽孢杆菌复制叉暂停时防止细胞分裂。
Mol Microbiol. 2010 Nov;78(4):866-82. doi: 10.1111/j.1365-2958.2010.07369.x. Epub 2010 Sep 23.
3
Biological characterization of novel inhibitors of the gram-positive DNA polymerase IIIC enzyme.革兰氏阳性DNA聚合酶IIIC酶新型抑制剂的生物学特性
Antimicrob Agents Chemother. 2005 Mar;49(3):987-95. doi: 10.1128/AAC.49.3.987-995.2005.
4
Properties of a Bacillus subtilis strain lacking DNA polymerase I.一株缺乏DNA聚合酶I的枯草芽孢杆菌菌株的特性
Nucleic Acids Res. 1974 Mar;1(3):461-77. doi: 10.1093/nar/1.3.461.
5
Prophage induction in thermosensitive DNA mutants of Bacillus subtilis.枯草芽孢杆菌温度敏感型DNA突变体中的原噬菌体诱导
Mol Gen Genet. 1984;194(3):451-6. doi: 10.1007/BF00425557.
6
Factors involved in the initiation of phage phi 29 DNA replication in vitro: requirement of the gene 2 product for the formation of the protein p3-dAMP complex.体外噬菌体φ29 DNA复制起始所涉及的因素：基因2产物对蛋白质p3-dAMP复合物形成的需求。
Nucleic Acids Res. 1983 Mar 11;11(5):1309-23. doi: 10.1093/nar/11.5.1309.
7
Formation of competent Bacillus subtilis cells.枯草芽孢杆菌感受态细胞的形成。
J Bacteriol. 1983 Feb;153(2):813-21. doi: 10.1128/jb.153.2.813-821.1983.
8
Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e.枯草芽孢杆菌DNA聚合酶III是烈性噬菌体phi e复制所必需的。
J Virol. 1974 Dec;14(6):1337-42. doi: 10.1128/JVI.14.6.1337-1342.1974.
9
Mechanism of inhibition of Bacillus subtilis DNA polymerase 3 by the arylhydrazinopyrimidine antimicrobial agents.芳基肼嘧啶类抗菌剂对枯草芽孢杆菌DNA聚合酶3的抑制机制。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2973-7. doi: 10.1073/pnas.71.8.2973.
10
Development of novel inhibitor probes of DNA polymerase III based on dGTP analogs of the HPUra type: base, nucleoside and nucleotide derivatives of N2-(3,4-dichlorobenzyl)guanine.基于HPUra型dGTP类似物的新型DNA聚合酶III抑制剂探针的开发：N2-(3,4-二氯苄基)鸟嘌呤的碱基、核苷和核苷酸衍生物
Nucleic Acids Res. 1990 Dec 25;18(24):7381-7. doi: 10.1093/nar/18.24.7381.
本文引用的文献
1
2
3
Codon--anticodon pairing: the wobble hypothesis.密码子-反密码子配对：摆动假说
J Mol Biol. 1966 Aug;19(2):548-55. doi: 10.1016/s0022-2836(66)80022-0.
4
Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.6-（对羟基苯偶氮）尿嘧啶对细菌DNA复制的抑制作用：对枯草芽孢杆菌修复和半保留合成的不同影响
J Mol Biol. 1971 Jul 14;59(1):1-16. doi: 10.1016/0022-2836(71)90409-8.
5
6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis.6-（对羟基苯偶氮）尿嘧啶：噬菌体感染的枯草芽孢杆菌中宿主DNA复制的选择性抑制剂。
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1454-61. doi: 10.1073/pnas.67.3.1454.
6
7
Inhibition of E. coli DNA polymerase II by ara-CTP.阿糖胞苷三磷酸对大肠杆菌DNA聚合酶II的抑制作用。
Nat New Biol. 1971 Dec 29;234(52):286-8. doi: 10.1038/newbio234286a0.
8
Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
9
Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.无细胞提取物中的纯化及DNA合成：DNA聚合酶II的特性
Proc Natl Acad Sci U S A. 1971 Apr;68(4):761-4. doi: 10.1073/pnas.68.4.761.
10
A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1.大肠杆菌的一种新的DNA聚合酶活性。I. 大肠杆菌polA1中存在的该活性的纯化及特性
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1557-64. doi: 10.1016/0006-291x(70)90565-6.
Zotero 插件