Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Universidade Federal de Rondônia, Porto Velho, Rondônia, Brazil.
Mycopathologia. 2022 Aug;187(4):375-384. doi: 10.1007/s11046-022-00644-7. Epub 2022 Jul 1.
Feline sporotrichosis has emerged as an important public health issue in some countries, especially Brazil. Currently, zoonotic transmission of Sporothrix brasiliensis by domestic cats is the major sporotrichosis spread form throughout this country. Sporotrichosis in Brazil is a good model for the One Health concept application, which connects the environment, human and animal health. Under this thinking, the aim of this study was to investigate the seroprevalence of sporotrichosis in cats from Rolim de Moura, Rondônia, Brazil, using antibody detection by an ELISA test previously validated for human diagnosis. For the standardization of this test, 30 serum samples from cats with proven sporotrichosis and 11 sera from healthy cats were used. The assay showed 87% sensitivity and 100% specificity for the diagnosis of feline sporotrichosis. After the standardization, 202 serum samples from distinct cats from Rolim de Moura were evaluated. The test was positive in 63 (31.19%) cats from the studied area. A multivariate analysis revealed that living far from forest or agricultural areas as well as pure breed animals had higher odds ratios (3.157 and 2.281, respectively) for the presence of detectable levels of anti-Sporothrix antibodies. These results show the applicability of this assay in the detection of anti-Sporothrix antibodies in feline serum samples and point to a putative new occurrence area of urban sporotrichosis dispersing to the North region of Brazil.
猫传染性孢子丝菌病在一些国家，特别是巴西，已成为一个重要的公共卫生问题。目前，由家猫传播的巴西芽生菌引起的人畜共患病传播是该国主要的孢子丝菌病传播形式。巴西的孢子丝菌病是应用“同一健康”理念的一个很好的范例，它将环境、人类和动物健康联系起来。基于这一理念，本研究旨在调查巴西朗多尼亚州罗利姆德莫拉（Rolim de Moura）地区猫的孢子丝菌病血清流行率，使用先前经人类诊断验证的 ELISA 试验进行抗体检测。为了规范该检测，使用 30 份经证实患有孢子丝菌病的猫血清样本和 11 份健康猫血清进行检测。该检测对猫孢子丝菌病的诊断具有 87%的敏感性和 100%的特异性。在标准化该检测后，评估了来自罗利姆德莫拉的 202 份不同猫的血清样本。检测结果显示，研究地区的 63 只（31.19%）猫呈阳性。多变量分析显示，生活在远离森林或农业区以及纯种动物的猫具有更高的检测到抗孢子丝菌抗体的可能性（比值比分别为 3.157 和 2.281）。这些结果表明该检测方法可应用于检测猫血清样本中的抗孢子丝菌抗体，并提示巴西北部地区可能出现新的城市孢子丝菌病散发区。
