Doutorado da Rede Nordeste de Biotecnologia (RENORBIO)-Universidade Católica de Pernambuco (UNICAP) Recife, PE, Brazil.
Molecules. 2010 Oct 8;15(10):6931-40. doi: 10.3390/molecules15106931.
A new strain of Serratia marcescens UCP1459 isolated from a semi-arid soil produced the natural red pigment prodigiosin, characterized by an uncommon pyrrolylpyrromethane skeleton. Prodigiosin is a promising drug due to its reported antifungal, immunosuppressive and anti-proliferative activities. The objective of this work was to indentify a suitable medium to simultaneously enhance S. marcescens growth and pigment production using renewable resources obtained from industrial wastes. S. marcescens produced the highest level of prodigiosin (49.5 g/L) at 48 h of cultivation using 6% "manipueira" (cassava wastewater) supplemented with mannitol (2%) at pH 7 and 28 °C. Carbohydrates in "manipueira" and mannitol play a role in the enhanced cell growth and prodigiosin production. The purified pigment extracted from the biomass was analyzed by mass spectrophotometry and showed the expected molecular weight of 324 Da corresponding to prodigiosin. In conclusion, we have successfully designed a new, economically feasible medium supporting enhanced S. marcescens growth and a high yield production of prodigiosin.
一株从半干旱土壤中分离到的粘质沙雷氏菌 UCP1459 菌株能够产生天然红色色素灵菌红素，其特征是具有不常见的吡咯并吡咯烷骨架。灵菌红素由于具有抗真菌、免疫抑制和抗增殖活性，因此是一种很有前途的药物。本研究的目的是使用从工业废物中获得的可再生资源，确定一种合适的培养基，同时提高粘质沙雷氏菌的生长和色素产量。在 pH 值为 7、28°C 的条件下，利用 6%“manipueira”（木薯废水）和甘露醇（2%）作为补充物，粘质沙雷氏菌在 48 小时的培养中产生了最高水平的灵菌红素（49.5 g/L）。“manipueira”和甘露醇中的碳水化合物在促进细胞生长和灵菌红素生产中发挥了作用。从生物质中提取的纯化色素通过质谱分析，显示出预期的分子量为 324 Da，对应于灵菌红素。总之，我们成功设计了一种新的、经济可行的培养基，支持粘质沙雷氏菌的生长和灵菌红素的高产。
