Sun Shi-Qing, Wang Yu-Jie, Xu Wei, Zhu Chang-Jun, Liu Xiao-Xia
a College of Biological, Chemical Sciences and Engineering , Jiaxing University , Jiaxing , China.
Prep Biochem Biotechnol. 2015;45(2):101-8. doi: 10.1080/10826068.2013.877029.
Prodigiosin extraction from dried Serratia marcescens jx1 cells using ultrasound-assisted extraction was optimized. The experiment was carried out in accordance with a central composite design (CCD) three-level and single-variable approach. The extraction time, extraction temperature, and solute to solvent ratio with the application of ultrasonication were optimized using response surface methodology (RSM) to maximize the extraction of prodigiosin from dried S. marcescens jx1 cells. The response of prodigiosin was determined using spectrophotometry. A quadratic model was established to predict the prodigiosin extraction yield. The analysis of variance showed that the quadratic model significantly contributed to the response of prodigiosin. The optimal extraction parameters were an extraction time of 17.5 min, an extraction temperature of 23.4°C, and a solvent-to-solute ratio of 1:27.2. Under these optimum conditions, the average prodigiosin yield was 4.3 g±0.02 g from 100 g of dried cells, which matches the predicted values. The obtained optimum conditions for prodigiosin extraction provide a scientific basis for the economical large-scale production of prodigiosin.
对采用超声辅助萃取法从干燥的粘质沙雷氏菌jx1细胞中提取灵菌红素的工艺进行了优化。实验按照中心复合设计（CCD）的三级单变量方法进行。利用响应面法（RSM）对超声处理时的萃取时间、萃取温度和溶质与溶剂的比例进行了优化，以实现从干燥的粘质沙雷氏菌jx1细胞中最大程度地提取灵菌红素。采用分光光度法测定灵菌红素的响应值。建立了二次模型来预测灵菌红素的提取产率。方差分析表明，二次模型对灵菌红素的响应有显著贡献。最佳萃取参数为：萃取时间17.5分钟、萃取温度23.4℃、溶剂与溶质的比例为1:27.2。在这些最佳条件下，从100克干燥细胞中获得的灵菌红素平均产量为4.3克±0.02克，与预测值相符。所获得的灵菌红素提取最佳条件为灵菌红素的经济大规模生产提供了科学依据。
