基于成像的细胞器膜和脂质分析的化学生物学工具。
Chemical biology tools for imaging-based analysis of organelle membranes and lipids.
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan; ERATO, Japan Science and Technology Agency (JST), 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
出版信息
Curr Opin Chem Biol. 2022 Oct;70:102182. doi: 10.1016/j.cbpa.2022.102182. Epub 2022 Jun 29.
Membrane biology studies have revealed that in addition to providing structural support for compartment formation and membrane protein function, subcellular biomembranes are also critically involved in many biological events. To facilitate our understanding of the functions, biophysical properties and structural dynamics of organelle membranes, various exciting chemical biology tools have recently emerged. This short review aims to describe the latest molecular probes for organelle membrane studies. In particular, we will feature chemical strategies to visualize and quantitatively analyze the dynamic propeties of organelle membranes and lipids and discuss current limitations and potential future directions of this challenging research area.
膜生物学研究表明，除了为隔室形成和膜蛋白功能提供结构支持外，亚细胞生物膜还在许多生物事件中起着至关重要的作用。为了帮助我们理解细胞器膜的功能、生物物理特性和结构动态，最近出现了各种令人兴奋的化学生物学工具。本文简要综述了细胞器膜研究的最新分子探针，特别介绍了用于可视化和定量分析细胞器膜和脂质动态特性的化学策略，并讨论了这一具有挑战性的研究领域的当前局限性和潜在的未来方向。
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