Department of Oral Medicine, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Eur J Med Res. 2022 Jul 2;27(1):108. doi: 10.1186/s40001-022-00735-7.
Bacteria associated with dental caries have a high ability to produce organic acids from dietary carbohydrates during growth and metabolism under acidic conditions. In contrast, many symbiotic bacteria produce ammonia through the arginine deiminase (ADS) system, which modulates the pH of the oral cavity. L-Arginine metabolism by ADS is a significant inhibitor in the progression of tooth decay. This study aimed to investigate the effect of L-arginine on growth, biofilm formation, and antibiotic susceptibility in Streptococcus mutans.
In this study, the effect of L-arginine in different concentrations on the growth rate, antibiotic susceptibility, and inhibition of biofilm formation in S. mutans was investigated.
The bacterial exponential growth rate was enhanced by 100 μM L-arginine (P > 0.05). The growth inhibition zone diameter of CAZ, CTR, AMP, and AMC-Clav antibiotics was reduced after 24 h of exposure in the presence of various concentrations of L-arginine specifically at 100 μM. L-Arginine also enhanced biofilm development at 5 and 10 μM concentrations, but reduced it at 50 and 100 μM concentrations.
According to the results of the present study, optimization of L-arginine concentration and its use as an adjunctive therapy or in combination with mouthwash or varnish is recommended to prevent oral caries.
与龋齿相关的细菌在生长和代谢过程中具有在酸性条件下从膳食碳水化合物中产生有机酸的高能力。相比之下，许多共生细菌通过精氨酸脱亚氨酶（ADS）系统产生氨，从而调节口腔的 pH 值。ADS 对 L-精氨酸的代谢是龋齿进展的重要抑制剂。本研究旨在研究 L-精氨酸对变形链球菌生长、生物膜形成和抗生素敏感性的影响。
本研究中，研究了不同浓度的 L-精氨酸对变形链球菌生长速率、抗生素敏感性和生物膜形成抑制的影响。
100 μM L-精氨酸（P>0.05）可增强细菌指数生长率。在存在不同浓度的 L-精氨酸的情况下，经过 24 小时的暴露，CAZ、CTR、AMP 和 AMC-Clav 抗生素的生长抑制区直径减小，特别是在 100 μM 时。L-精氨酸在 5 和 10 μM 浓度下也增强了生物膜的发展，但在 50 和 100 μM 浓度下则降低了生物膜的发展。
根据本研究的结果，建议优化 L-精氨酸浓度并将其用作辅助治疗或与漱口液或牙釉质结合使用，以预防口腔龋齿。
