Shang Peng, Dong Shixiong, Han Yuqing, Bo Suxue, Ye Yourong, Duan Mengqi, Chamba Yangzom
College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet, People's Republic of China; The Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, People's Republic of China.
College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet, People's Republic of China; The Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, People's Republic of China.
Chemosphere. 2022 Nov;307(Pt 2):135558. doi: 10.1016/j.chemosphere.2022.135558. Epub 2022 Jun 30.
The gut microbiota can change to varying degrees because of changes in the environment. In the present study, we performed microbial amplicon sequencing on the feces of people who had long-term exposure to swine farms (F) and that of people living in normal environments (S) to investigate the impact of the environment on the human gut microbiota. A total of 1,283,503 high-quality ordered sequences were obtained, which provided different levels of microbial classification and statistics. We found that different environments did not alter the richness and diversity of the microbial communities in participants, but caused significant changes in the proportion of some bacteria. The main bacterial phyla found in group F participants were Firmicutes (69.44-89.03%), Actinobacteria (1.7-18.95%), and Bacteroidetes (1.17-22.35%); those found in group S participants were Firmicutes (49.93-95.04%), Bacteroidetes (0.62-39.59%), and Proteobacteria (0.98-11.95%). Additionally, because of changes in phylum proportions, the Bugbase phenotypic classification predicted an increase in the proportion of Gram-positive bacteria in group F and an increase in the proportion of Gram-negative bacteria in group S. In conclusion, our findings suggest that human exposure to swine farms can reshape the gut microbiota, resulting in changes in the microbial abundances. This change can potentially reduce the odds of developing bowel disease and contribute to the prevention of intestinal diseases, providing a theoretical basis for improving human health.
由于环境变化，肠道微生物群会发生不同程度的改变。在本研究中，我们对长期接触养猪场的人群（F组）和生活在正常环境中的人群（S组）的粪便进行了微生物扩增子测序，以研究环境对人体肠道微生物群的影响。共获得1,283,503条高质量的有序序列，这些序列提供了不同水平的微生物分类和统计数据。我们发现，不同环境并未改变参与者微生物群落的丰富度和多样性，但导致了一些细菌比例的显著变化。F组参与者中主要的细菌门类为厚壁菌门（69.44 - 89.03%）、放线菌门（1.7 - 18.95%）和拟杆菌门（1.17 - 22.35%）；S组参与者中主要的细菌门类为厚壁菌门（49.93 - 95.04%）、拟杆菌门（0.62 - 39.59%）和变形菌门（0.98 - 11.95%）。此外，由于门类比例的变化，Bugbase表型分类预测F组革兰氏阳性菌比例增加，S组革兰氏阴性菌比例增加。总之，我们的研究结果表明，人类接触养猪场会重塑肠道微生物群，导致微生物丰度发生变化。这种变化可能会降低患肠道疾病的几率，有助于预防肠道疾病，为改善人类健康提供理论依据。
