State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Planta. 2022 Jul 4;256(2):29. doi: 10.1007/s00425-022-03941-z.
Preferential expression of OsPLC1 is detected at the heading stage of rice, OsPLC1 overexpression results in early flowering, increased-grain size and yield; however, opposing phenotypes produced in the osplc1 mutants. Abstract: The importance of phospholipase C (PLC) in plant development has been demonstrated in several studies. OsPLC1, a member of PI-PLC in rice, although its role in the response to salt stress of rice seedlings has been reported, its functions in the growth and development of rice is elusive. Here, we report that OsPLC1 expression could be detectable in various tissues throughout the developmental stages of rice, and the highest expression level of OsPLC1 was detected at the heading stage. OsPLC1 overexpression (OE) produced rice plants with early flowering, whereas OsPLC1 loss-of-function led to delay in flowering. The expression levels of subset genes, which are involved in the control of flowering time in rice, were altered in the plants of OE and osplc1. In addition, the enlargement of grain size was observed in OE plants, however, the reduction of grain size was noticed in osplc1 mutants. The increase in the grain size and the grain yield of OE lines were associated with the improvement of cell length and expression levels of a set of genes related to cell expansion, contrarily, the decrease in osplc1 mutant grain size and yield were linked to declined cell length and expression levels of related genes. No significant differences, in terms of the grain quality of mature seeds, were found in OE and osplc1 mutants, with compared to those in Nipponbare (Nip). In summary, our study suggests that OsPLC1 could modulate rice flowering time and grain size.
OsPLC1 在水稻抽穗期优先表达，OsPLC1 过表达导致早花、增加粒大小和产量；然而，osplc1 突变体产生相反的表型。摘要：已有多项研究表明磷脂酶 C (PLC) 在植物发育中的重要性。OsPLC1 是水稻 PI-PLC 的成员，尽管其在水稻幼苗对盐胁迫的反应中的作用已有报道，但它在水稻生长和发育中的功能仍不清楚。在这里，我们报告 OsPLC1 的表达可以在水稻发育的各个阶段的各种组织中检测到，并且 OsPLC1 的最高表达水平在抽穗期检测到。OsPLC1 过表达 (OE) 产生了早花的水稻植株，而 OsPLC1 功能丧失导致开花延迟。参与水稻开花时间控制的亚组基因的表达水平在 OE 和 osplc1 植物中发生改变。此外，在 OE 植物中观察到粒大小的增大，然而，在 osplc1 突变体中观察到粒大小的减小。OE 系粒大小和粒产量的增加与细胞长度的增加和一组与细胞扩展相关的基因的表达水平有关，相反，osplc1 突变体粒大小和产量的减少与细胞长度的下降和相关基因的表达水平降低有关。与 Nipponbare (Nip) 相比，OE 和 osplc1 突变体成熟种子的粒质量没有显著差异。总之，我们的研究表明 OsPLC1 可以调节水稻的开花时间和粒大小。
