College of Life Science, Northwest Normal University, Lanzhou, 730070, China.
Planta. 2024 Oct 22;260(6):121. doi: 10.1007/s00425-024-04554-4.
AtPLC1 plays a critical role in plant growth, development, and response to drought stress. Phosphoinositide-specific phospholipase C (PI-PLC) hydrolyzes substrates to generate secondary messengers crucial for plant growth, development, and stress responses. Drought escape (DE) response is an adaptive strategy that plants employ under drought conditions. The expression levels of the flower meristem-specific gene APETALA 1 and flowering regulatory genes FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 were downregulated in plc1, and FLOWERING LOCUS C was upregulated. The flowering time of the plc1flc double mutant was earlier than that of the wild type. Transcriptome analysis revealed that the Gene Ontology of differentially expressed genes (DEGs) was enriched in abscisic acid (ABA) response signaling, and Kyoto Encyclopedia of Genes and Genomes analysis revealed differential gene expression annotated to plant hormone signaling pathways. Our experiments show that AtPLC1 is upregulated by ABA in Arabidopsis. Under ABA induction and water stress, wild-type plants exhibit a DE response, and the DE response in plc1 disappears. Expression levels of ABA signaling pathway transcription factors ABA-responsive element-binding factors 3 (ABF3) and ABF4 were downregulated in plc1. In conclusion, our study suggests that AtPLC1 participates in regulating plant growth and development and participates in the DE response through the regulation of ABA signaling pathway transcription factors ABF3/ABF4. The study enhances our comprehension of the role of AtPLC1 in plant development and drought stress, providing a theoretical foundation for further investigation into DE responses.
AtPLC1 在植物生长、发育和对干旱胁迫的响应中起着关键作用。磷酸肌醇特异性磷脂酶 C（PI-PLC）水解底物，生成对植物生长、发育和应激反应至关重要的第二信使。干旱逃避（DE）反应是植物在干旱条件下采用的一种适应策略。花分生组织特异性基因 APETALA 1 和开花调节基因 FLOWERING LOCUS T 和 SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 的表达水平在 plc1 中下调，而 FLOWERING LOCUS C 上调。plc1flc 双突变体的开花时间早于野生型。转录组分析表明，差异表达基因（DEGs）的基因本体论富集在脱落酸（ABA）响应信号中，京都基因与基因组百科全书分析显示，差异基因表达注释到植物激素信号通路。我们的实验表明，ABA 在拟南芥中上调 AtPLC1。在 ABA 诱导和水分胁迫下，野生型植物表现出 DE 反应，而 plc1 中的 DE 反应消失。ABA 信号通路转录因子 ABA 响应元件结合因子 3（ABF3）和 ABF4 的表达水平在 plc1 中下调。综上所述，我们的研究表明，AtPLC1 通过调节 ABA 信号通路转录因子 ABF3/ABF4 参与植物生长和发育的调控，并参与 DE 反应。该研究增强了我们对 AtPLC1 在植物发育和干旱胁迫中作用的理解，为进一步研究 DE 反应提供了理论基础。
