Abbaszadeh Somayeh, Teimouri Aref, Mahmoudi Mohammad Reza, Roushan Zahra Atrkar, Hajipour Nayereh, Majidi-Shad Bijan, Sharifdini Meysam
Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Food Waterborne Parasitol. 2022 Jun 3;27:e00166. doi: 10.1016/j.fawpar.2022.e00166. eCollection 2022 Jun.
Detection of in chicken products indicates risk of transmission to consumers. The objective of the current study was to investigate the molecular prevalence of in free-ranging and industrial chickens in Guilan province, Northern Iran. A total of 150 chicken heart samples including 75 free-range and 75 industrial chickens were collected from farmers' markets and chicken retailers in Guilan, Northern Iran, between October 2017 and August 2018. Genomic DNA were extracted from samples and examined for evidence of using polymerase chain reaction (PCR) targeting the B1 gene. The B1-positive samples were further analyzed by nested-PCR for SAG1 gene. Of the 150 samples, DNA fragments were detected in 59 (39.3%), including 30 (40%) free-range and 29 (38.7%) industrial chicken. No significant differences of DNA detection was observed between the free-range and industrial chicken samples ( = 0.73). Four selected positive samples were used for amplifying and sequencing of the SAG1 gene. The results revealed that all four sequences of SAG1 had 100% similarity with sequences previously isolated from an AIDS/HIV patient in Mazandaran province, Northern Iran. Furthermore, the phylogenetic analysis demonstrated that all four sequences were closely related to Type I of . However, our Type I identification is preliminary and needs to be confirmed by further multilocus sequence typing (MLST) analysis. The findings of the present study provide new data about the presence of DNA in chicken hearts in the study area. These results confirm that chicken can be used as sentinels for environment contamination; however, further studies are needed to determine the viability of in chicken hearts from Iran for risk assessment.
鸡肉制品中检测到[具体病原体名称未给出]表明存在传播给消费者的风险。本研究的目的是调查伊朗北部吉兰省散养和工业化养殖鸡中[具体病原体名称未给出]的分子流行情况。2017年10月至2018年8月期间,从伊朗北部吉兰省的农贸市场和鸡肉零售商处共采集了150份鸡心脏样本,其中包括75份散养鸡和75份工业化养殖鸡的样本。从样本中提取基因组DNA,并使用针对B1基因的聚合酶链反应(PCR)检测[具体病原体名称未给出]的证据。对B1阳性样本进一步进行巢式PCR检测SAG1基因。在150个样本中,59个(39.3%)检测到[具体病原体名称未给出]DNA片段,其中包括30个(40%)散养鸡和29个(38.7%)工业化养殖鸡。散养鸡和工业化养殖鸡样本之间[具体病原体名称未给出]DNA检测无显著差异(P = 0.73)。选取4个阳性样本进行SAG1基因的扩增和测序。结果显示,所有4个SAG1序列与之前从伊朗北部马赞德兰省一名艾滋病/艾滋病毒患者分离出的[具体病原体名称未给出]序列具有100%的相似性。此外,系统发育分析表明,所有4个序列与[具体病原体名称未给出]的I型密切相关。然而,我们的I型鉴定是初步的,需要通过进一步的多位点序列分型(MLST)分析来确认。本研究结果提供了有关研究区域鸡心脏中[具体病原体名称未给出]DNA存在情况的新数据。这些结果证实鸡可作为环境污染的哨兵;然而,需要进一步研究来确定伊朗鸡心脏中[具体病原体名称未给出]的生存能力以进行风险评估。