Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies et Biotechnologie (IM2B), Aix-Marseille Université - Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 7255, Marseille, France.
Université de Limoges, INSERM, Centre Hospitalier Universitaire (CHU) Limoges, Unité Mixte de Recherche (UMR) 1092, Limoges, France.
Front Cell Infect Microbiol. 2022 Jun 16;12:859181. doi: 10.3389/fcimb.2022.859181. eCollection 2022.
Bacteria of the genus are environmental germs, with an unknown reservoir. It can become opportunistic pathogens in immunocompromised patients, causing bacteremia, meningitis, pneumonia, or peritonitis. In recent years, has emerged with increasing incidence in patients with cystic fibrosis (CF). Recent studies showed that is involved in the degradation of the respiratory function of patients with CF. The respiratory ecosystem of patients with CF is colonized by bacterial species that constantly fight for space and access to nutrients. The type VI secretion system (T6SS) empowers this constant bacterial antagonism, and it is used as a virulence factor in several pathogenic bacteria. This study aimed to investigate the prevalence of the T6SS genes in isolated in patients with CF. We also evaluated clinical and molecular characteristics of T6SS-positive strains. We showed that possesses a T6SS gene cluster and that some environmental and clinical isolates assemble a functional T6SS nanomachine T6SS is used to target competing bacteria, including other CF-specific pathogens. Finally, we demonstrated the importance of the T6SS in the internalization of in lung epithelial cells and that the T6SS protein Hcp is detected in the sputum of patients with CF. Altogether, these results suggest for the first time a role of T6SS in CF-lung colonization by and opens promising perspective to target this virulence determinant as innovative theranostic options for CF management.
属于环境细菌，其储存库未知。它可以在免疫功能低下的患者中成为机会性病原体，引起菌血症、脑膜炎、肺炎或腹膜炎。近年来，在囊性纤维化（CF）患者中， 的发病率不断上升。最近的研究表明， 参与 CF 患者呼吸功能的下降。CF 患者的呼吸生态系统被不断争夺空间和获取营养的细菌物种定植。VI 型分泌系统（T6SS）赋予了这种持续的细菌拮抗作用，并被用作几种病原菌的毒力因子。本研究旨在调查囊性纤维化患者中分离的 中 T6SS 基因的流行情况。我们还评估了 T6SS 阳性 株的临床和分子特征。我们表明 拥有 T6SS 基因簇，一些环境和临床分离株组装了一个功能性的 T6SS 纳米机器。T6SS 用于靶向竞争细菌，包括其他 CF 特异性病原体。最后，我们证明了 T6SS 在 内化入肺上皮细胞中的重要性，并且在 CF 患者的痰液中检测到 T6SS 蛋白 Hcp。总之，这些结果首次表明 T6SS 在 通过 CF 肺定植中的作用，并为将这一毒力决定因素作为 CF 管理的创新治疗选择提供了有前景的视角。
