Occurrence of spp. in backyard poultry in Bosnia and Herzegovina.

BACKGROUND

Infected poultry is one of the most important reservoirs of .

AIMS

The investigation presented here was conducted to examine the occurrence of in fecal samples among selected flocks of backyard poultry in Bosnia and Herzegovina (B&H).

METHODS

Isolation and identification of was performed in accordance with BAS EN ISO 6579/AMD 1:2007. When genus was confirmed, the determination of the antigenic formula of isolates was performed in accordance with BAS CEN ISO/TR 6579-3:2015. After that, serotypes were subjected to antibiotic susceptibility testing using EUVSEC sensititre microtiter plates impregnated with different concentrations of antibiotics. At the end, real-time PCR was used to detect extended spectrum β-lactamases (ESBL) and carbapeneamase encoding genes ( , , , , , , , and ).

RESULTS

S spp. was detected in pooled feces from four backyards, housed by chickens only. Three isolates were confirmed by slide agglutination as serotype Enteritidis and one as serotype Typhimurium. Antibiotic susceptibility testing by microdilution did not reveal phenotypical resistance among these four isolates. Real-time PCR used to detect ESBL and carbapeneamase encoding genes revealed the gene in one Enteritidis isolate.

CONCLUSION

Data presented in this study provide further evidence on the circulation of different serotypes in backyard poultry in B&H. These findings emphasize the potential role of backyard poultry in the epidemiology of salmonellosis and the risks it poses for keepers, consumers, and general public health.