Zhou Tongxi, Niu Mengfan, Liu Ruichen, Li Li
College of Sports and Human Sciences, Harbin Sport University, Harbin, China.
Graduate Faculty, Harbin Sport University, Harbin, China.
Front Genet. 2022 Jun 16;13:901827. doi: 10.3389/fgene.2022.901827. eCollection 2022.
This work aimed to study the intervention effect of exercise on lipid metabolism in NAFLD rats, provide a more scientific experimental basis for exploring and improving the theoretical system of exercise intervention in NAFLD, and further provide a theoretical research basis for clinical treatment of NAFLD. Forty healthy male Sprague Dawley rats were randomly divided into a blank control group (BC,14) and a model group (MO, 26). After 6°weeks of modeling, the MO group was randomly divided into the model control group (MC, 12) and the aerobic exercise group (AE, 12). Platform running intervention in group E was conducted at a slope of 0°, a speed of 15 m/min, 1 h/time, once a day, six times a week, and a day of rest, for 8°weeks in total. After the intervention, the liver tissues of rats were taken for pathological sections, and serum was taken and analyzed for TC, TG, LDL-C, HDL-C, and FFA levels. Under the light microscope, the liver tissue structure of rats in the BC group was complete and clear, the structure of liver lobules was clear and normal, the volume of hepatocytes was uniform, the nucleus was in the middle, and the cytoplasm was red-stained, and no steatosis of hepatocytes was found. The liver of rats in the MC group showed diffuse fatty lesions, disordered structure of hepatic lobules, disordered arrangement of hepatic cords, different sizes of hepatocytes, loose cytoplasm, and diffuse lipid droplets of different sizes in the cytoplasm. The accumulation of liver lipid droplets in the AE group was improved compared with the MC group, the number of fat vacuoles in hepatocytes was significantly reduced, and the degree of liver lipid deposition was reduced. Compared with the BC group, the content of TC, TG, LDL-C, and FFA in the serum of the MC group increased significantly ( < 0.01), and the content of HDL-C decreased significantly ( < 0.01). Compared with the MC group, the content of TC, TG, LDL-C, and FFA in the serum of the AE group decreased significantly ( < 0.01/ < 0.05), and the content of HDL-C increased significantly ( < 0.01). Therefore, moderate-intensity aerobic exercise has an intervention effect on lipid metabolism in NAFLD rats, which can be used as one of the means to treat NAFLD.
本研究旨在探讨运动对非酒精性脂肪性肝病（NAFLD）大鼠脂质代谢的干预作用，为完善NAFLD运动干预理论体系提供更科学的实验依据，进而为NAFLD的临床治疗提供理论研究基础。将40只健康雄性Sprague Dawley大鼠随机分为空白对照组（BC组，14只）和模型组（MO组，26只）。造模6周后，将MO组随机分为模型对照组（MC组，12只）和有氧运动组（AE组，12只）。AE组进行平台跑步干预，坡度为0°，速度为15米/分钟，每次1小时，每天1次，每周6次，休息1天，共干预8周。干预结束后，取大鼠肝脏组织进行病理切片，并采集血清检测总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）和游离脂肪酸（FFA）水平。光镜下，BC组大鼠肝组织结构完整、清晰，肝小叶结构清晰、正常，肝细胞体积均匀，细胞核位于中央，细胞质呈红色，未发现肝细胞脂肪变性。MC组大鼠肝脏呈现弥漫性脂肪病变，肝小叶结构紊乱，肝索排列紊乱，肝细胞大小不一，细胞质疏松，细胞质内可见大小不等的弥漫性脂滴。与MC组相比，AE组肝脏脂滴堆积情况有所改善，肝细胞内脂肪空泡数量明显减少，肝脏脂质沉积程度减轻。与BC组相比，MC组大鼠血清中TC、TG、LDL-C和FFA含量显著升高（P<0.01），HDL-C含量显著降低（P<0.01）。与MC组相比，AE组大鼠血清中TC、TG、LDL-C和FFA含量显著降低（P<0.01/P<0.05），HDL-C含量显著升高（P<0.01）。因此，中等强度有氧运动对NAFLD大鼠脂质代谢具有干预作用，可作为治疗NAFLD的手段之一。
