Wu Yu-Hsueh, Chiang Chun-Pin
Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Institute of Oral Medicine, School of Dentistry, National Cheng Kung University, Tainan, Taiwan.
J Dent Sci. 2022 Jul;17(3):1201-1205. doi: 10.1016/j.jds.2022.04.008. Epub 2022 Apr 22.
BACKGROUND/PURPOSE: Virtual microscopy has been used for learning and teaching histology and pathology laboratory course for more than 10 years. This study aimed to compare the learning outcome of virtual microscopy with that of real light microscopy in oral histology laboratory course among dental students.
The study included twenty-three undergraduate dental students who took the oral histology laboratory course. The evaluation methods of the study were divided into two parts: questionnaire survey (6 questions) and histological diagnosis ability assessment tests. The data were collected and analyzed statistically.
Dental students showed a significantly higher acceptance rate of virtual microscopy than that of real light microscopy for learning oral histology laboratory course (all -values < 0.01). Moreover, dental students using virtual microscopy with digitized virtual slides for learning oral histology laboratory course possessed significantly better histological diagnosis ability than those using real light microscopy with traditional glass slides for learning oral histology laboratory course ( < 0.001).
Virtual microscopy with digitized virtual slides seems to be a great method to learn oral histology laboratory course. Based on the results of our study, we believe that the virtual microscopy with digitized virtual slides may gradually replace the real light microscopy with traditional glass slides for learning and teaching oral histology laboratory course. We foresee that virtual microscopy with digitized microscopic images will be extensively applied in many fields in the near future.
背景/目的：虚拟显微镜已用于组织学和病理学实验室课程的教学十余年。本研究旨在比较牙科学生在口腔组织学实验室课程中使用虚拟显微镜与实体光学显微镜的学习效果。
本研究纳入了23名修读口腔组织学实验室课程的本科牙科学生。研究的评估方法分为两部分：问卷调查（6个问题）和组织学诊断能力评估测试。收集数据并进行统计学分析。
牙科学生在学习口腔组织学实验室课程时，对虚拟显微镜的接受率显著高于实体光学显微镜（所有P值<0.01）。此外，使用数字化虚拟切片的虚拟显微镜学习口腔组织学实验室课程的牙科学生，其组织学诊断能力明显优于使用传统玻璃切片的实体光学显微镜学习口腔组织学实验室课程的学生（P<0.001）。
数字化虚拟切片的虚拟显微镜似乎是学习口腔组织学实验室课程的好方法。基于我们的研究结果，我们认为数字化虚拟切片的虚拟显微镜可能会逐渐取代传统玻璃切片的实体光学显微镜，用于口腔组织学实验室课程的教学。我们预计，带有数字化显微图像的虚拟显微镜在不久的将来将广泛应用于许多领域。
