口腔微生物群对龈沟液中抗瓜氨酸化蛋白抗体IgA存在情况的影响。
Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid.
作者信息
de Smit Menke J, Rahajoe Poerwati Soetji, Raveling-Eelsing Elisabeth, Lisotto Paola, Harmsen Hermie J M, Kertia Nyoman, Vissink Arjan, Westra Johanna
机构信息
Department of Dentistry, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.
Department of Oral and Maxillofacial Surgery, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.
出版信息
Front Oral Health. 2022 Jun 16;3:904711. doi: 10.3389/froh.2022.904711. eCollection 2022.
INTRODUCTION
The relation between rheumatoid arthritis (RA) and periodontitis (PD) has been investigated ever since the discovery of the citrullinating enzyme peptidyl arginine deaminase presents in the oral bacterium . Recently, we demonstrated the presence of RA autoantibodies, especially of IgA anti-citrullinated protein antibody (ACPA), in gingival crevicular fluid (GCF) of Indonesian patients with and without RA or PD which might indicate the local formation of RA antibodies in the periodontium.
AIM
The purpose of this study was to assess whether the subgingival microbiome is related to the presence of IgA ACPA in the GCF of healthy individuals with or without PD.
PATIENTS AND METHODS
Healthy individuals with a known periodontal status and high IgA ACPA (>0.1 U/ml) in GCF ( = 27) were selected and matched for age, gender, periodontal status, and smoking status with 27 healthy individuals without IgA ACPA in their GCF. Taxonomic profiling of the subgingival microbiome was based on bacterial 16S rRNA gene sequencing. Downstream analyses were performed to assess compositional differences between healthy subjects with or without IgA ACPA in GCF and with or without PD.
RESULTS
Between groups with or without PD, or with or without IgA ACPA in GCF, no differences in alpha diversity were seen. Beta diversity was different between groups with or without PD ( < 0.0001), and a trend was seen in subjects with PD between subjects with or without IgA ACPA in GCF ( = 0.084). Linear discriminant analysis effect size (LEfSe) revealed no significant differences in the total population between subjects with IgA ACPA compared to subjects without IgA ACPA in GCF. Although was not identified by LEfSe, its relative abundance was significantly higher in healthy individuals with high IgA ACPA in GCF compared to individuals without IgA ACPA in GCF ( = 0.0363). Zooming in on the subgroup with PD, LEfSe revealed that species , and were more abundant in the subjects with IgA ACPA in GCF compared to subjects without IgA ACPA in GCF.
CONCLUSION
Periodontitis and certain taxa, including , seem to be associated with the local presence of ACPA in the periodontium.
引言
自从发现口腔细菌中存在瓜氨酸化酶肽基精氨酸脱氨酶以来，类风湿性关节炎（RA）与牙周炎（PD）之间的关系就一直受到研究。最近，我们在患有和未患有RA或PD的印度尼西亚患者的龈沟液（GCF）中证实了RA自身抗体的存在，尤其是IgA抗瓜氨酸化蛋白抗体（ACPA），这可能表明牙周组织中存在局部形成的RA抗体。
目的
本研究的目的是评估龈下微生物群是否与有无PD的健康个体的GCF中IgA ACPA的存在有关。
患者与方法
选择龈沟液中牙周状况已知且IgA ACPA水平较高（>0.1 U/ml）的健康个体（n = 27），并在年龄、性别、牙周状况和吸烟状况方面与27名龈沟液中无IgA ACPA的健康个体进行匹配。龈下微生物群的分类分析基于细菌16S rRNA基因测序。进行下游分析以评估龈沟液中有或无IgA ACPA以及有或无PD的健康受试者之间的组成差异。
结果
在有或无PD的组之间，或龈沟液中有或无IgA ACPA的组之间，α多样性未见差异。有或无PD的组之间β多样性不同（P < 0.0001），在患有PD的受试者中，龈沟液中有或无IgA ACPA的受试者之间有一个趋势（P = 0.084）。线性判别分析效应大小（LEfSe）显示，龈沟液中有IgA ACPA的受试者与无IgA ACPA的受试者在总体人群中无显著差异。尽管LEfSe未识别出[具体物种名称未给出]，但其相对丰度在龈沟液中IgA ACPA水平较高的健康个体中显著高于龈沟液中无IgA ACPA的个体（P = 0.0363）。聚焦于患有PD的亚组，LEfSe显示，与龈沟液中无IgA ACPA的受试者相比，龈沟液中有IgA ACPA的受试者中[具体物种名称未给出]、[具体物种名称未给出]和[具体物种名称未给出]的丰度更高。
结论
牙周炎和某些分类群，包括[具体物种名称未给出]，似乎与牙周组织中ACPA的局部存在有关。
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