Zhao Lei, Wang Lei, Guo Zimeng, Zhang Ning, Feng Qisheng, Li Bo
School of Graduation, Changchun University of Chinese Medicine, Changchun, China.
School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, China.
Front Pharmacol. 2022 Jun 16;13:933669. doi: 10.3389/fphar.2022.933669. eCollection 2022.
Two polysaccharide fractions from (Blanco) Benth. () (designated as PCB-1 and PCB2-1) were isolated by water extraction and purified by Sepharose chromatography. The chemical properties of the polysaccharides were characterised, and their antioxidant activities were evaluated. The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively. The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively. Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios. The polysaccharides exhibited significantly high antioxidant activities based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities. experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver. These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
从（布兰科）本特（）中分离出两种多糖级分（分别命名为PCB - 1和PCB2 - 1），通过水提取法获得，并经琼脂糖凝胶色谱法纯化。对这些多糖的化学性质进行了表征，并评估了它们的抗氧化活性。粗多糖（PCB）、PCB - 1和PCB2 - 1的糖含量分别为58.74%、90.23%和88.61%。PCB - 1和PCB2 - 1的分子量分别测定为97.8 kDa和12.8 kDa。单糖组成分析表明，这三种多糖均由甘露糖、鼠李糖、半乳糖醛酸、半乳糖、葡萄糖和阿拉伯糖组成，但摩尔比不同。基于对羟基自由基的清除活性、金属离子螯合能力和铁还原能力，这些多糖表现出显著的高抗氧化活性。在氧化损伤小鼠模型中的实验表明，PCB - 1提高了抗氧化酶的水平，包括超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶，并抑制了血清和肝脏中丙二醛的形成。这些发现表明，PCB - 1作为功能性食品中的抗氧化剂具有巨大潜力。
