Zhang Wei, Chen Rui, Xu Xiong, Zhu Liang, Liu Yanbin, Yu XiaoJie, Tang GuoKe
Joint Surgery Department, Zhuzhou Central Hospital, Zhuzhou, China.
Department of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, China.
Front Pharmacol. 2022 Jun 16;13:922032. doi: 10.3389/fphar.2022.922032. eCollection 2022.
In tissue engineering, hydrogel scaffolds allow various cells to be cultured and grown and then implanted to repair or replace the damaged areas. Here in this work, kartogenin (KGN), an effectively chondro-inductive non-protein bioactive drug molecule, was incorporated into a composite hydrogel comprising the positively charged chitosan (CS) and methacrylated gelatin (GelMA) polymers to fabricate appropriate microenvironments of bone marrow mesenchymal stem cells (BMSCs) for cartilage regeneration. Based on the combination of physical chain entanglements and chemical crosslinking effects, the resultant GelMA-CS@KGN composite hydrogels possessed favorable network pores and mechanical strength. cytotoxicity showed the excellent biocompatibility for facilitating the cell growth, adhesion, proliferation, and differentiation. The long-term sustainable KGN release from the hydrogel scaffolds promoted the chondrogenic differentiation that can be employed as an alternative candidate for cartilage tissue regeneration.
在组织工程中，水凝胶支架可使各种细胞进行培养和生长，然后植入以修复或替换受损区域。在本研究中，将一种具有有效软骨诱导作用的非蛋白质生物活性药物分子卡托金（KGN）掺入由带正电荷的壳聚糖（CS）和甲基丙烯酸化明胶（GelMA）聚合物组成的复合水凝胶中，以构建适合骨髓间充质干细胞（BMSC）软骨再生的微环境。基于物理链缠结和化学交联作用的结合，所得的GelMA-CS@KGN复合水凝胶具有良好的网络孔隙和机械强度。细胞毒性表明其具有优异的生物相容性，有利于细胞的生长、黏附、增殖和分化。水凝胶支架中KGN的长期持续释放促进了软骨生成分化，可作为软骨组织再生的替代候选物。
