酶促转化为3-羟基犬尿氨酸后，用高效液相色谱法测定血清中的犬尿氨酸。
Determination of kynurenine in serum by high-performance liquid chromatography after enzymatic conversion to 3-hydroxykynurenine.
作者信息
Naito J, Ishiguro I, Murazumi T, Morimoto M
出版信息
Anal Biochem. 1987 Feb 15;161(1):16-9. doi: 10.1016/0003-2697(87)90644-0.
PMID:3578780
Abstract
A method for the determination of the physiological level of kynurenine in human serum based upon conversion of kynurenine to 3-hydroxykynurenine by enzymatic reaction with the mitochondrial fraction and NADPH and analysis by reversed-phase high-performance liquid chromatography with electrochemical detection is described. Tryptophan gave no interference. For one analysis, 0.2 ml of serum was sufficient, compared with the large volume (5.0 ml) required for other methods.
摘要
描述了一种基于色氨酸与线粒体部分和NADPH通过酶促反应转化为3 - 羟基色氨酸，并通过反相高效液相色谱 - 电化学检测进行分析来测定人血清中色氨酸生理水平的方法。色氨酸不产生干扰。与其他方法所需的大体积（5.0 ml）相比，一次分析0.2 ml血清就足够了。
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