Englander S W, Calhoun D B, Englander J J
Anal Biochem. 1987 Mar;161(2):300-6. doi: 10.1016/0003-2697(87)90454-4.
Published procedures for experimentation under anoxic conditions generally involve specialized apparatus that hinders the easy manipulation of experimental samples. We describe here some procedures that rapidly remove oxygen from experimental solutions, maintain anoxia with simple equipment for long periods of time, and do not interfere with normal sample addition and removal, spectrometric measurements, chromatographic manipulations, and the like. Anoxia can be achieved and maintained by the use of an enzyme system (glucose oxidase, glucose, catalase), or an inorganic oxygen-reducing system (ferrous pyrophosphate), or dithionite. Physical isolation of experimental samples from atmospheric oxygen can be maintained by continuous flushing with treated argon gas and/or by an overlay of heavy mineral oil.
已发表的在缺氧条件下进行实验的程序通常涉及专门的仪器，这会妨碍对实验样品的轻松操作。我们在此描述一些程序，这些程序能快速从实验溶液中去除氧气，使用简单设备长时间维持缺氧状态，并且不干扰正常的样品添加和移除、光谱测量、色谱操作等。通过使用酶系统（葡萄糖氧化酶、葡萄糖、过氧化氢酶）、无机氧还原系统（焦磷酸亚铁）或连二亚硫酸盐，可以实现并维持缺氧状态。通过用处理过的氩气连续冲洗和/或用重质矿物油覆盖，可以保持实验样品与大气氧气的物理隔离。
