Glucose Transport through -Acetylgalactosamine Phosphotransferase System in C Strain.

When , a glucose-specific phosphotransferase system (PTS) component, is deleted in , growth can be severely poor because of the lack of efficient glucose transport. We discovered a new PTS transport system that could transport glucose through the growth-coupled experimental evolution of -deficient C strain under anaerobic conditions. Genome sequencing revealed mutations in , which encodes a repressor of -acetylgalactosamine (Aga) PTS expression in evolved progeny strains. RT-qPCR analysis showed that the expression of Aga PTS gene increased because of the loss-of-function of . We confirmed the efficient Aga PTS-mediated glucose uptake by genetic complementation and anaerobic fermentation. We discussed the discovery of new glucose transporter in terms of different genetic backgrounds of strains, and the relationship between the pattern of mixed-acids fermentation and glucose transport rate.