Liu Shangshu, Zhao Haocheng, Zheng Mengyan, Wang Hongkai, Jing Chen, Zhang Weini, Hu Fengxiao
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Oct;260:109406. doi: 10.1016/j.cbpc.2022.109406. Epub 2022 Jul 4.
Sulfamethoxazole (SMX), a broad-spectrum antibiotic, has been widely used in the treatment and prevention of infection caused by bacteria in recent years. The present study was aimed to evaluate the response mechanisms to SMX stress in gills and digestive gland of Corbicula fluminea (O. F. Müller, 1774). To this end, clams were exposed to environmentally relevant concentrations of SMX (0, 1, 10 and 100 μg/L) for 7 and 28 days, and siphon behavior, tissue-specific enzymatic and transcriptional changes were assayed. Our results showed that exposure to SMX significantly suppressed filtration rate and acetylcholinesterase (AChE) activity, activated antioxidant defense system and elevated transcription of several genes related to cell apoptosis in gills and digestive gland of clams. In general, SMX at environmentally relevant concentrations exhibited a negative impact on siphon behavior and induced neurotoxicology, oxidative stress and cell apoptosis in C. fluminea. The current study will help broaden our understanding of the ecotoxicity of SMX on freshwater bivalves.
磺胺甲恶唑（SMX）是一种广谱抗生素，近年来已广泛用于治疗和预防细菌引起的感染。本研究旨在评估河蚬（Corbicula fluminea，O. F. Müller，1774）鳃和消化腺对SMX胁迫的反应机制。为此，将蛤蜊暴露于环境相关浓度的SMX（0、1、10和100μg/L）中7天和28天，并检测虹吸管行为、组织特异性酶和转录变化。我们的结果表明，暴露于SMX会显著抑制滤过率和乙酰胆碱酯酶（AChE）活性，激活抗氧化防御系统，并提高蛤蜊鳃和消化腺中与细胞凋亡相关的几个基因的转录水平。总体而言，环境相关浓度的SMX对虹吸管行为产生负面影响，并诱导河蚬出现神经毒理学、氧化应激和细胞凋亡。本研究将有助于拓宽我们对SMX对淡水双壳贝类生态毒性的理解。
