Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Environ Sci Pollut Res Int. 2023 Aug;30(38):88598-88611. doi: 10.1007/s11356-023-28702-7. Epub 2023 Jul 12.
Diclofenac (DCF), one of the most current and widely used nonsteroidal anti-inflammatory drugs (NSAIDs), has been frequently detected in aquatic environments worldwide. However, the ecotoxicological effects of DCF on freshwater invertebrates remain largely unknown. In the present study, Corbicula fluminea were exposed to environmentally relevant concentrations of DCF (0, 2, 20, and 200 μg/L) for 28 days, and the potential adverse effects of DCF on siphoning behavior, antioxidant responses, and apoptosis were investigated. Our results showed that the siphon efficiencies of clams were significantly suppressed under DCF stress. DCF exerted neurotoxicity via reducing the activity of acetylcholinesterase (AChE) in gills and digestive gland of C. fluminea. Exposure to DCF induced antioxidant stress and increased malondialdehyde (MDA) levels in both gills and digestive gland of C. fluminea. Transcriptional alterations of apoptosis-related genes indicated that DCF might induce apoptosis by triggering mitochondrial apoptotic pathway. These findings can improve our understanding of the ecological risk of DCF in freshwater ecosystems.
双氯芬酸（DCF）是目前应用最广泛的非甾体类抗炎药（NSAIDs）之一，在世界范围内的水生环境中频繁被检出。然而，DCF 对淡水无脊椎动物的生态毒理学效应在很大程度上尚不清楚。在本研究中，我们采用环境相关浓度（0、2、20 和 200μg/L）的 DCF 对河蚬（Corbicula fluminea）进行了 28 天的暴露实验，研究了 DCF 对河蚬虹吸行为、抗氧化反应和细胞凋亡的潜在不良影响。结果表明，在 DCF 胁迫下，河蚬的虹吸效率显著降低。DCF 通过降低河蚬鳃和消化腺中乙酰胆碱酯酶（AChE）的活性表现出神经毒性。暴露于 DCF 会诱导抗氧化应激，并增加河蚬鳃和消化腺中丙二醛（MDA）的水平。凋亡相关基因的转录变化表明，DCF 可能通过触发线粒体凋亡途径诱导细胞凋亡。这些发现可以提高我们对 DCF 在淡水生态系统中生态风险的认识。
