Yuan Hai Ying, Caron Carolyn T, Vandenberg Albert, Bett Kirstin E
Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Aquatic and Crop Resource Development Research Center, National Research Council of Canada, Saskatoon, SK, Canada.
Front Genet. 2022 Jun 20;13:891702. doi: 10.3389/fgene.2022.891702. eCollection 2022.
Lentil is an important pulse crop not only because of its high nutrient value but also because of its ecological advantage in a sustainable agricultural system. Our previous work showed that the cultivated lentil and wild lentil germplasm respond differently to light environments, especially to low R/FR-induced shade conditions. Little is known about how cultivated and wild lentils respond to shade at the level of gene expression and function. In this study, transcriptomic profiling of a cultivated lentil (Lupa, ) and a wild lentil (BGE 016880, ) at several growth stages is presented. transcriptomes were assembled for both genotypes, and differential gene expression analysis and gene ontology enrichment analysis were performed. The transcriptomic resources generated in this study provide fundamental information regarding biological processes and genes associated with shade responses in lentils. BGE 016880 and Lupa shared a high similarity in their transcriptomes; however, differential gene expression profiles were not consistent between these two genotypes. The wild lentil BGE 016880 had more differentially expressed genes than the cultivated lentil Lupa. Upregulation of genes involved in gibberellin, brassinosteroid, and auxin synthesis and signaling pathways, as well as cell wall modification, in both genotypes explains their similarity in stem elongation response under the shade. Genes involved in jasmonic acid and flavonoid biosynthesis pathways were downregulated in BGE 016880 only, and biological processes involved in defense responses were significantly enriched in the wild lentil BGE 016880 only. Downregulation of WRKY and MYB transcription factors could contribute to the reduced defense response in BGE 016880 but not in Lupa under shade conditions. A better understanding of shade responses of pulse crop species and their wild relatives will play an important role in developing genetic strategies for crop improvement in response to changes in light environments.
小扁豆是一种重要的豆类作物，不仅因其营养价值高，还因其在可持续农业系统中的生态优势。我们之前的研究表明，栽培小扁豆和野生小扁豆种质对光照环境的反应不同，尤其是对低红光/远红光诱导的遮荫条件。关于栽培小扁豆和野生小扁豆在基因表达和功能水平上如何响应遮荫，我们知之甚少。在本研究中，我们展示了一种栽培小扁豆（卢帕）和一种野生小扁豆（BGE 016880）在几个生长阶段的转录组分析。为这两个基因型组装了转录组，并进行了差异基因表达分析和基因本体富集分析。本研究中产生的转录组资源提供了有关小扁豆遮荫反应相关生物学过程和基因的基本信息。BGE 016880和卢帕的转录组具有高度相似性；然而，这两个基因型之间的差异基因表达谱并不一致。野生小扁豆BGE 016880比栽培小扁豆卢帕有更多差异表达基因。两种基因型中参与赤霉素、油菜素内酯和生长素合成及信号通路以及细胞壁修饰的基因上调，解释了它们在遮荫下茎伸长反应的相似性。仅在BGE 016880中，参与茉莉酸和类黄酮生物合成途径的基因下调，仅在野生小扁豆BGE 016880中，参与防御反应的生物学过程显著富集。WRKY和MYB转录因子的下调可能导致BGE 016880在遮荫条件下防御反应降低，但卢帕没有。更好地了解豆类作物及其野生近缘种的遮荫反应，将在制定应对光照环境变化的作物改良遗传策略中发挥重要作用。
