Department of Pharmacy Services, Nihat Delibalta Göle Vocational High School, Ardahan University, Ardahan, 75700, Turkey.
Central Research & Application Laboratory, Agri Ibrahim Cecen University, Agri, Turkey.
Chem Biodivers. 2022 Aug;19(8):e202200280. doi: 10.1002/cbdv.202200280. Epub 2022 Jul 7.
In the current study, some phenolic compounds, including acteoside, isoacteoside, echinacoside, and arenarioside purified and characterized from Plantago subulata. These compounds were tested for its antioxidant potential, including Fe and Cu reductive ability and Fe chelating effects. The inhibitory effects of isolated phenolic compounds were tested towards human carbonic anhydrase I and II isoenzymes (hCA I and hCA II), butyrylcholinesterase (BChE) acetylcholinesterase (AChE), aldose reductase (AR) and α-glycosidase (α-gly). K values were found these compounds in range of 0.24±0.05-1.38±0.34 μM against hCA I, 0.194±0.018-1.03±0.06 μM against hCA II, 0.043±0.01-0.154±0.02 μM against AChE, 3.92±1.08-11.93±4.45 μM against BChE, 0.082±0.0008-1.68±0.42 μM against AR, and 6.93±2.74-17.17±6.70 μM against α-glycosidase. As a result, isolated compounds displayed inhibition effects against studied all metabolic enzymes. They are promising candidates for treating disorders like Alzheimer's disease, diabetes mellitus, glaucoma, leukemia, and epilepsy.
在本研究中，从车前草中分离和鉴定了一些酚类化合物，包括毛蕊花糖苷、异毛蕊花糖苷、麦角甾苷和苍术苷。这些化合物的抗氧化能力进行了测试，包括 Fe 和 Cu 的还原能力和 Fe 螯合作用。还测试了分离得到的酚类化合物对人碳酸酐酶 I 和 II 同工酶（hCA I 和 hCA II）、丁酰胆碱酯酶（BChE）、乙酰胆碱酯酶（AChE）、醛糖还原酶（AR）和 α-糖苷酶（α-gly）的抑制作用。发现这些化合物对 hCA I 的 K 值在 0.24±0.05-1.38±0.34 μM 范围内，对 hCA II 的 K 值在 0.194±0.018-1.03±0.06 μM 范围内，对 AChE 的 K 值在 0.043±0.01-0.154±0.02 μM 范围内，对 BChE 的 K 值在 3.92±1.08-11.93±4.45 μM 范围内，对 AR 的 K 值在 0.082±0.0008-1.68±0.42 μM 范围内，对 α-糖苷酶的 K 值在 6.93±2.74-17.17±6.70 μM 范围内。结果表明，分离得到的化合物对研究的所有代谢酶都有抑制作用。它们是治疗阿尔茨海默病、糖尿病、青光眼、白血病和癫痫等疾病的有前途的候选药物。
