PG & Research Department of Zoology, Pachaiyappa's College, Chennai, 600 030, Tamil Nadu, India.
PG & Research Department of Chemistry, Pachaiyappa's College, Chennai, 600 030, Tamil Nadu, India.
Appl Biochem Biotechnol. 2022 Dec;194(12):5607-5626. doi: 10.1007/s12010-022-04048-7. Epub 2022 Jul 7.
The present study was designed to isolate a potential compound from the extracts of Elytraria acaulis (E. acaulis) for ovarian cancer. n-Hexane, ethyl acetate, chloroform, acetone and methanol extract were taken using the Soxhlet method. Thin layer, column chromatography, NMR and MASS studies were done for the isolation and structural characterization of the compound. Finally, the novel compound (Z)-3-(2-methyl-3-oxoprop-1-en-1-yl) phenyl heptanoate was identified. MTT assay, cell morphology and cell cycle analysis were done to evaluate the anticancer property of the compound. In the MTT assay, the percentage of the cell viability treated with the isolated compound was decreased while increasing the concentration of the compound. Cancer cells treated with the isolated compound showed distinct morphological changes when compared to the control untreated cells. In the cell cycle analysis, the isolated compound induced a significant increase in the percentage of cells in G0/G1 phase and a decrease in the percentage of cells in the S phase and G2-M phase of the PA 1 cell lines. The cell cycle arrest induced by the isolated compound may account for its antiproliferative capacity. Hence, the novel compound isolated from E. acaulis can be a potent candidate in the designing of anticancer drugs.
本研究旨在从 Elytraria acaulis（E. acaulis）提取物中分离出一种潜在的化合物，用于治疗卵巢癌。采用索氏提取法提取正己烷、乙酸乙酯、氯仿、丙酮和甲醇提取物。通过薄层层析、柱层析、NMR 和 MASS 研究对化合物进行分离和结构鉴定。最终鉴定出了一种新型化合物（Z）-3-(2-甲基-3-氧代丙-1-烯-1-基)苯基庚酸酯。通过 MTT 测定、细胞形态学和细胞周期分析评估化合物的抗癌特性。在 MTT 测定中，随着化合物浓度的增加，用分离得到的化合物处理的细胞活力百分比降低。与未经处理的对照细胞相比，用分离得到的化合物处理的癌细胞表现出明显的形态变化。在细胞周期分析中，分离得到的化合物诱导 PA1 细胞系中 G0/G1 期细胞的百分比显著增加，S 期和 G2-M 期细胞的百分比减少。分离得到的化合物诱导的细胞周期停滞可能是其抗增殖能力的原因。因此，从 Elytraria acaulis 中分离出的新型化合物可能是设计抗癌药物的潜在候选物。
