Ghagane Shridhar C, Puranik Sridevi I, Kumbar Vijay M, Nerli Rajendra B, Jalalpure Sunil S, Hiremath Murigendra B, Neelagund Shivayogeeswar, Aladakatti Ravindranath
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India.
Department of Biotechnology, K.L.E's R. L. Science Institute, (Autonomous), Belagavi, India.
Integr Med Res. 2017 Mar;6(1):79-87. doi: 10.1016/j.imr.2017.01.004. Epub 2017 Jan 16.
To determine the phytochemical constituents, antioxidant, and anticancer activities of leaf extracts on DU-145 and PC-3 human prostate cancer cell lines.
Leaf sample was subjected to Soxhlet extraction method with increasing polarity of solvents, namely, chloroform, ethyl acetate, methanol, ethanol, and aqueous. Phytochemical screening was done using different biochemical tests. Quantitative analysis for phenol was determined by Folin-Ciocalteu reagent method. The antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl, ferric ion reducing power assay, and phosphomolybdenum assay. anticancer activity on DU-145 and PC-3 human prostate cancer cell lines was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay.
Phytochemical screening confirmed the presence of phyto-constituents like alkaloids, flavonoids, glycosides, phenols, lignins, saponins, sterols, tannins, anthraquinone, and reducing sugar. Methanol and ethanol extracts exhibited higher phenolic content as compare to aqueous extract. Antioxidant capacities were shown highest in methanol and ethanol extracts based on the test performed. The methanol and ethanol leaf extracts were found to be selectively cytotoxic to (DU-145 and PC-3) prostate cancer cell lines with IC values 529.44 ± 42.07 μg/mL and 677.11 ± 37.01 μg/mL for DU-145 and 547.55 ± 33.52 μg/mL and 631.99 ± 50.24 μg/mL for PC-3 respectively, while it had no cytotoxic effect on normal mice embryo fibroblast cells.
The results indicate that was a promising antioxidant and anticancer agent for DU-145 and PC-3 human prostate cancer cell lines. However, further studies are needed to conclude its therapeutic use.
确定叶提取物对DU - 145和PC - 3人前列腺癌细胞系的植物化学成分、抗氧化和抗癌活性。
采用索氏提取法，使用极性递增的溶剂（即氯仿、乙酸乙酯、甲醇、乙醇和水）对叶样本进行提取。通过不同的生化试验进行植物化学筛选。采用福林 - 西奥尔特试剂法测定酚类的定量分析。使用2,2 - 二苯基 - 1 - 苦基肼、铁离子还原能力测定法和磷钼酸测定法测试抗氧化活性。通过（3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑）MTT法评估对DU - 145和PC - 3人前列腺癌细胞系的抗癌活性。
植物化学筛选证实存在生物碱、黄酮类、糖苷、酚类、木质素、皂苷、甾醇、单宁、蒽醌和还原糖等植物成分。与水提取物相比，甲醇和乙醇提取物表现出更高的酚含量。基于所进行测试，甲醇和乙醇提取物的抗氧化能力最高。发现甲醇和乙醇叶提取物对（DU - 145和PC - 3）前列腺癌细胞系具有选择性细胞毒性，DU - 145的IC值分别为529.44 ± 42.07 μg/mL和677.11 ± 37.01 μg/mL，PC - 3的IC值分别为547.55 ± 33.52 μg/mL和631.99 ± 50.24 μg/mL，而对正常小鼠胚胎成纤维细胞无细胞毒性作用。
结果表明该提取物对DU - 145和PC - 3人前列腺癌细胞系是一种有前景的抗氧化和抗癌剂。然而，需要进一步研究以确定其治疗用途。
