非编码 RNA 对核小体稳定性影响的评估方法。
Method for Evaluating Effects of Non-coding RNAs on Nucleosome Stability.
机构信息
Laboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2022;2509:195-208. doi: 10.1007/978-1-0716-2380-0_12.
In eukaryotic cells, genomic DNA is stored in the nucleus in a structure called chromatin. The nucleosome, the basic structural unit of chromatin consisting of DNA wound around a histone octamer, regulates access of transcription machinery to DNA. Nucleosome stability is thus tightly associated with gene expression. Recently, a class of non-coding RNAs was found to be directly associated with chromatin. Although these non-coding RNAs are reportedly important in genome regulation, the molecular mechanisms through which these RNAs act remain unclear. Here, we introduce a biochemical method to evaluate the effects of ncRNAs on nucleosome stability, using the breast cancer-associated ncRNA Eleanor2 as an example. This method is useful for assessing the effects of different RNAs on chromatin stability and conformation.
在真核细胞中，基因组 DNA 存储在细胞核中一种称为染色质的结构中。核小体是染色质的基本结构单位，由 DNA 缠绕在组蛋白八聚体周围组成，调节转录机制与 DNA 的相互作用。因此，核小体的稳定性与基因表达密切相关。最近，人们发现一类非编码 RNA 与染色质直接相关。尽管这些非编码 RNA 在基因组调控中据报道很重要，但这些 RNA 发挥作用的分子机制仍不清楚。在这里，我们介绍了一种生化方法来评估 ncRNA 对核小体稳定性的影响，以乳腺癌相关 ncRNA Eleanor2 为例。该方法可用于评估不同 RNA 对染色质稳定性和构象的影响。
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