墨累谷脑炎病毒、西尼罗河病毒、日本脑炎病毒和圣路易斯脑炎病毒所指定蛋白质的比较。

Comparison of proteins specified by Murray Valley encephalitis, West Nile, Japanese encephalitis and St. Louis encephalitis viruses.

作者信息

Wright P J, Warr H M

出版信息

Aust J Exp Biol Med Sci. 1986 Oct;64 ( Pt 5):485-8. doi: 10.1038/icb.1986.52.

DOI:10.1038/icb.1986.52

Abstract

The relationships among proteins specified by Murray Valley encephalitis (MVE), West Nile (WN), Japanese encephalitis (JE) and St. Louis encephalitis (SLE) viruses were examined by peptide mapping. [3H]methionine-labelled tryptic peptides of viral proteins were separated by reverse phase high performance liquid chromatography (HPLC) and the separation profiles for a given protein specified by the different viruses were compared. Analyses of the non-structural protein NV5 (P98 or NS5) suggested that WN and SLE were the most closely related pair of viruses, and that JE was the virus most distant from the other three. Analyses of the structural proteins C and E failed to show the close relationship between WN and SLE indicated by the NV5 results, but did suggest that NV5 was the most conserved and E the least conserved of the three proteins.

摘要

通过肽图谱分析研究了墨累谷脑炎（MVE）病毒、西尼罗河（WN）病毒、日本脑炎（JE）病毒和圣路易斯脑炎（SLE）病毒所指定的蛋白质之间的关系。用反相高效液相色谱法（HPLC）分离病毒蛋白的[³H]甲硫氨酸标记胰蛋白酶肽，并比较不同病毒所指定的给定蛋白质的分离图谱。对非结构蛋白NV5（P98或NS5）的分析表明，WN和SLE是关系最密切的一对病毒，而JE是与其他三种病毒关系最远的病毒。对结构蛋白C和E的分析未能显示出NV5结果所表明的WN和SLE之间的密切关系，但确实表明NV5是这三种蛋白质中最保守的，而E是最不保守的。