通过肽图分析对库京病毒、西尼罗河病毒和墨累谷脑炎病毒所指定的蛋白质进行比较。

Comparisons by peptide mapping of proteins specified by Kunjin, West Nile and Murray Valley encephalitis viruses.

作者信息

Wright P J, Warr H M, Westaway E G

出版信息

Aust J Exp Biol Med Sci. 1983 Dec;61 ( Pt 6):641-53. doi: 10.1038/icb.1983.61.

DOI:10.1038/icb.1983.61

Abstract

The relationships among virus-specified proteins of Murray Valley encephalitis (MVE), Kunjin (KUN) and West Nile (WN) viruses were investigated by peptide mapping of exhaustive proteolytic digests of radioactively labelled polypeptides. Maps of the three structural proteins (E, C and M) derived from purified virions and of two non-structural proteins (NV5 and NV4) obtained from infected cells were compared. For each polypeptide considered, the peptide maps of the KUN and WN virus-specified proteins were more similar to each other than either was to the map of the corresponding MVE virus-specified protein. Since the polypeptides considered together account for approximately 60% of the coding capacity of the flavivirus genome, our results suggested that, for the three viruses examined, the genomes of KUN and WN viruses are the most closely related.

摘要

通过对放射性标记多肽的彻底蛋白水解消化产物进行肽图谱分析，研究了墨累谷脑炎病毒（MVE）、库京病毒（KUN）和西尼罗河病毒（WN）的病毒特异性蛋白之间的关系。比较了从纯化病毒粒子中获得的三种结构蛋白（E、C和M）以及从感染细胞中获得的两种非结构蛋白（NV5和NV4）的图谱。对于所研究的每种多肽，KUN病毒和WN病毒特异性蛋白的肽图谱彼此之间比它们与相应MVE病毒特异性蛋白的图谱更相似。由于所研究的多肽合起来约占黄病毒基因组编码能力的60%，我们的结果表明，在所检测的三种病毒中，KUN病毒和WN病毒的基因组关系最为密切。