AEP1 介导的 PNA-蛋白缀合能够实现膜蛋白的可擦除成像。
AEP1-mediated PNA-protein conjugation enables erasable imaging of membrane proteins.
机构信息
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu, 210023, P. R. China.
State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, Jiangsu, 210023, P. R. China.
出版信息
Chem Commun (Camb). 2022 Jul 26;58(60):8448-8451. doi: 10.1039/d2cc02153f.
We report the use of a protein ligase to covalently ligate a protein to a peptide nucleic acid (PNA). The rapid ligation demands only an N-terminal GL dipeptide in the target protein and a C-terminal NGL tripeptide in the PNA. We demonstrate the versatility of this approach by attaching a PNA strand to three different proteins. Lastly, we show that erasable imaging of EGFR on HEK293 cell membranes is achieved with DNA origami nanostructures and toehold-mediated strand displacement.
我们报告了使用一种蛋白质连接酶将蛋白质共价连接到肽核酸 (PNA) 上。这种快速连接只需要目标蛋白中的 N 端 GL 二肽和 PNA 中的 C 端 NGL 三肽。我们通过将 PNA 链连接到三种不同的蛋白质上证明了这种方法的多功能性。最后，我们表明使用 DNA 折纸纳米结构和引发介导的链置换可以实现对 HEK293 细胞膜上 EGFR 的可擦除成像。
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