尼日利亚拉各斯四个学术医疗中心的伤寒患者中伤寒血清型菌株携带的超广谱β-内酰胺酶编码基因。
Extended-spectrum Beta-lactamases Encoding Genes among serovar Typhi Isolates in Patients with Typhoid Fever from four Academic Medical Centers Lagos, Nigeria.
机构信息
Department of Microbiology, Faculty of Science, Lagos State University, Ojo, Lagos, Nigeria.
Department of Biology, College of Science, University of Babylon, Babylon, Hilla City, Iraq.
出版信息
Rev Invest Clin. 2022;74(3):165-171. doi: 10.24875/RIC.22000078.
BACKGROUND
There is scarce information about the occurrence of extended-spectrum β-lactamases (ESBLs) in a serovar Typhi () from patients with typhoid fever.
OBJECTIVE
To study the antimicrobial resistance and ESBL encoding genes among isolates in aforesaid patients from Lagos, Nigeria.
METHODS
isolates were collected from blood samples of typhoid fever patients from 4 academic medical centers in Lagos, Nigeria. The identification of isolates and their antibiotic susceptibility testing were performed by standard bacteriological techniques and disc diffusion method, respectively. The production of ESBLs was investigated using combination disk test (CDT) and polymerase chain reaction (PCR).
RESULTS
A total of 27 isolates was collected. All isolates were susceptible to imipenem and nitrofurantoin. Fifteen (55.6%) isolates were multidrug-resistant (MDR). The CDT test showed 11 (40.7%) ESBL producer isolates. However, the PCR revealed a higher occurrence rate for ESBL producers (66.7%, n = 18/27). The ESBL genes were as follows: (37.0%, n = 10/27), (18.5%, n = 5/27), and (44.4%, n = 12/27). All ESBL positive isolates were MDR.
CONCLUSIONS
This study showed the emergence of ESBL-harboring in patients with typhoid fever from Nigeria.
背景
从伤寒患者中分离出的伤寒血清型 Typhi()中，很少有关于产超广谱β-内酰胺酶（ESBLs）的信息。
目的
研究尼日利亚拉各斯 4 家学术医疗中心的伤寒患者中 分离株的抗菌药物耐药性和 ESBL 编码基因。
方法
从尼日利亚拉各斯的 4 家学术医疗中心的伤寒发热患者的血液样本中采集 分离株。通过标准细菌学技术和纸片扩散法分别进行分离株鉴定和药敏试验。使用联合纸片试验（CDT）和聚合酶链反应（PCR）检测 ESBL 的产生。
结果
共采集了 27 株分离株。所有分离株均对亚胺培南和呋喃妥因敏感。15 株（55.6%）分离株为多重耐药（MDR）。CDT 试验显示 11 株（40.7%）为 ESBL 产酶株。然而，PCR 显示 ESBL 产酶株的发生率更高（66.7%，n=18/27）。ESBL 基因如下： （37.0%，n=27）、 （18.5%，n=27）和 （44.4%，n=27）。所有 ESBL 阳性 分离株均为 MDR。
结论
本研究表明，尼日利亚伤寒患者中出现了携带 ESBL 的 。
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