Jin Yuhan, Ouyang Yina, Fan Xinyang, Huang Jing, Guo Wenbo, Miao Yongwang
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
Yunnan Animal Science and Veterinary Institute, Kunming 650224, China.
Anim Biosci. 2022 Nov;35(11):1656-1665. doi: 10.5713/ab.22.0120. Epub 2022 Jun 30.
Water buffalo, an important domestic animal in tropical and subtropical regions, play an important role in agricultural economy. It is an important source for milk, meat, horns, skin, and draft power, especially its rich milk that is the great source of cream, butter, yogurt, and many cheeses. In recent years, long noncoding RNAs (lncRNAs) have been reported to play pivotal roles in many biological processes. Previous studies for the mammary gland development of water buffalo mainly focus on protein coding genes. However, lncRNAs of water buffalo remain poorly understood, and the regulation relationship between mammary gland development/milk production traits and lncRNA expression is also unclear.
Here, we sequenced 22 samples of the milk somatic cells from three lactation stages and integrated the current annotation and identified 7,962 lncRNA genes.
By comparing the lncRNA genes of the water buffalo in the early, peak, and late different lactation stages, we found that lncRNA gene lnc-bbug14207 displayed significantly different expression between early and late lactation stages. And lnc-bbug14207 may regulate neighboring milk fat globule-EGF factor 8 (MFG-E8) and hyaluronan and proteoglycan link protein 3 (HAPLN3) protein coding genes, which are vital for mammary gland development.
This study provides the first genome-wide identification of water buffalo lncRNAs and unveils the potential lncRNAs that impact mammary gland development.
水牛是热带和亚热带地区重要的家畜，在农业经济中发挥着重要作用。它是牛奶、肉类、牛角、皮革和役力的重要来源，尤其是其丰富的乳汁是奶油、黄油、酸奶和许多奶酪的重要原料。近年来，长链非编码RNA（lncRNAs）已被报道在许多生物学过程中发挥关键作用。以往关于水牛乳腺发育的研究主要集中在蛋白质编码基因上。然而，水牛的lncRNAs仍知之甚少，乳腺发育/产奶性状与lncRNA表达之间的调控关系也不清楚。
在此，我们对三个泌乳阶段的22个乳体细胞样本进行了测序，并整合了当前的注释，鉴定出7962个lncRNA基因。
通过比较水牛在泌乳早期、高峰期和晚期不同阶段的lncRNA基因，我们发现lncRNA基因lnc-bbug14207在泌乳早期和晚期之间表现出显著不同的表达。并且lnc-bbug14207可能调控邻近的乳脂肪球-表皮生长因子8（MFG-E8）和透明质酸及蛋白聚糖连接蛋白3（HAPLN3）蛋白质编码基因，这些基因对乳腺发育至关重要。
本研究首次对水牛lncRNAs进行了全基因组鉴定，并揭示了影响乳腺发育的潜在lncRNAs。
