Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2023 Sep 1;24(17):13585. doi: 10.3390/ijms241713585.
The mammary glands, responsible for milk secretion, are regulated at a local level by various hormones, growth factors, non-coding RNAs, and other elements. Recent research has discovered the presence of lncRNAs in these glands, with suggestions that they may be essential for the maintenance and function of mammary glands. Besides directly controlling the gene and protein expression, lncRNAs are believed to play a significant part in numerous physiological and pathological processes. This study focused on examining the mammary gland tissues of Chinese Holstein cows, to identify and categorize long non-coding RNAs (lncRNAs). The research intended to distinguish lncRNAs in the mammary tissues of Holstein cows and contrast them between lactation and non-lactation periods. In this study, mammary gland tissues were sampled from three Holstein cows in early lactation ( = 3, 30 days postpartum) and non-lactation ( = 3, 315 days postpartum) on a large dairy farm in Jiangsu province. Mammary tissue samples were collected during early lactation and again during non-lactation. In total, we detected 1905 lncRNAs, with 57.3% being 500 bp and 612 intronic lncRNAs. The exon count for lncRNAs varied from 2 to 10. It was observed that 96 lncRNA expressions markedly differed between the two stages, with 83 genes being upregulated and 53 downregulated. Enrichment analysis results revealed that Gene Ontology (GO) analysis was primarily abundant in cellular processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that target genes were predominantly abundant in metabolic pathways, fatty acid biosynthesis, the immune system, and glycosphingolipid biosynthesis. This study analyzed the expression profile and characteristics of lncRNAs in the mammary gland tissues of Holstein cows during both lactation and non-lactation stages, forming a foundation for further investigation into the functional roles of lncRNAs in Holstein cows throughout lactation.
乳腺负责乳汁分泌，其功能受各种激素、生长因子、非编码 RNA 和其他因素的局部调节。最近的研究发现长链非编码 RNA（lncRNA）存在于这些腺体中，提示它们可能对乳腺的维持和功能至关重要。除了直接调控基因和蛋白质表达外，lncRNA 还被认为在许多生理和病理过程中发挥重要作用。本研究专注于检测中国荷斯坦奶牛的乳腺组织，以鉴定和分类长链非编码 RNA（lncRNA）。研究旨在区分荷斯坦奶牛乳腺组织中的 lncRNA，并比较泌乳期和非泌乳期之间的差异。在这项研究中，从江苏省一个大型奶牛场的 3 头泌乳早期（=3，产后 30 天）和非泌乳期（=3，产后 315 天）的荷斯坦奶牛中采集乳腺组织样本。在泌乳早期和非泌乳期分别采集乳腺组织样本。总共检测到 1905 个 lncRNA，其中 57.3%的长度为 500bp，612 个为内含子 lncRNA。lncRNA 的外显子数量从 2 到 10 不等。结果观察到，在两个阶段之间，96 个 lncRNA 的表达明显不同，其中 83 个基因上调，53 个基因下调。富集分析结果表明，GO 分析主要集中在细胞过程中。KEGG 通路分析表明，靶基因主要富集在代谢途径、脂肪酸生物合成、免疫系统和糖脂生物合成途径中。本研究分析了荷斯坦奶牛泌乳期和非泌乳期乳腺组织中 lncRNA 的表达谱和特征，为进一步研究 lncRNA 在荷斯坦奶牛泌乳期的功能作用奠定了基础。
