Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning Province; Engineering Research Center of Natural Medicine Active Molecule Research & Development, Liaoning Province; Key Laboratory of Natural Bioactive Compounds Discovery & Modification, Shenyang; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.
Fitoterapia. 2022 Sep;161:105250. doi: 10.1016/j.fitote.2022.105250. Epub 2022 Jul 4.
Three undescribed acylated sucroses (1-3), one undescribed butenolide analog (4) along with three known compounds (5-7) were isolated from the aqueous EtOH extract of the dried leaves of Tripterygium wilfordii. Their structures were elucidated on the basis of spectroscopic analyses, electron circular dichroism (ECD) techniques, and saccharide hydrolysis. All the isolated compounds were tested for their anti-tyrosinase effects. Among them, 6 exhibited similar inhibitory effects on tyrosinase with IC values of 0.073 mM comparing to arbutin. Additionally, the possible mechanism of the interaction between 6 and the active site of tyrosinase was explored by molecular docking.
从干燥的雷公藤叶的水乙醇提取物中分离得到三种未被描述的酰化蔗糖（1-3），一种未被描述的丁烯内酯类似物（4）以及三种已知化合物（5-7）。基于光谱分析、电子圆二色性（ECD）技术和糖水解，确定了它们的结构。所有分离得到的化合物均进行了酪氨酸酶抑制活性测试。其中，6 号化合物对酪氨酸酶的抑制活性与熊果苷相当，IC 值为 0.073 mM。此外，通过分子对接研究了 6 号化合物与酪氨酸酶活性中心相互作用的可能机制。
