BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain; Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country, UPV/EHU, Barrio Sarriena, s/n, 48940 Leioa, Spain.
BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain; Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country, UPV/EHU, Barrio Sarriena, s/n, 48940 Leioa, Spain.
Int J Biol Macromol. 2022 Sep 1;216:291-302. doi: 10.1016/j.ijbiomac.2022.07.004. Epub 2022 Jul 5.
Biocompatible and biodegradable hydrogels with biomimetic properties, such as self-repairing, are increasingly interesting for biomedical applications, particularly when they can be printed or in situ formed to mimic extracellular matrix or as personalized implantable devices in tissue regeneration or drug delivery. Photocrosslinkable hydrogels based on methacrylated chitosan (CHIMe) and hyaluronic acid that exhibit according with their composition, tuneable physico-chemical properties are here presented. The study of the conversion, gelation time, mechanical and rheological properties of photopolymerized CHIMe showed an optimal phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) initiator feed (0.1 % w). These photocrosslinkable hydrogels demonstrated being able to promote doubly crosslinked hydrogels with similar Young Moduli regardless the cycles of self-healing processes, and tailored swelling (25-70 swelling factor), mechanical (1 × 10-2 × 10 MPa) and rheological properties, as a function of polysaccharides relative content. Clear evidences have been found that fast photopolymerization of CHIMe/HA solutions leads to biocompatible (>80 % cell viability), biodegradable (20-24 days in hydrolytic medium) and robust self-healable hydrogels suitable for advanced biomedical and tissue engineering applications.
具有仿生特性的生物相容性和可生物降解水凝胶，例如自修复水凝胶，越来越受到生物医学应用的关注，特别是当它们可以打印或原位形成以模拟细胞外基质或作为组织再生或药物输送的个性化植入物时。本文介绍了基于甲基丙烯酰化壳聚糖（CHIMe）和透明质酸的光交联水凝胶，其具有根据组成可调的物理化学性质。对光聚合 CHIMe 的转化率、凝胶时间、机械和流变性能的研究表明，最优的苯甲酰基膦（LAP）引发剂进料为 0.1%（w）。这些光交联水凝胶表现出能够促进双交联水凝胶，其杨氏模量相似，而无论自修复过程的循环次数如何，并且可以调整溶胀度（25-70 溶胀因子）、机械性能（1×10-2×10 MPa）和流变性能，这取决于多糖的相对含量。已经有明确的证据表明，CHIMe/HA 溶液的快速光聚合导致生物相容性（>80%细胞活力）、可生物降解（在水解介质中 20-24 天）和坚固的自修复水凝胶，适用于先进的生物医学和组织工程应用。
