Maiz-Fernández Sheila, Pérez-Álvarez Leyre, Silván Unai, Vilas-Vilela José Luis, Lanceros-Méndez Senentxu
Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country, UPV/EHU, Barrio Sarriena, s/n, 48940 Leioa, Spain.
BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain.
Gels. 2022 Jul 29;8(8):477. doi: 10.3390/gels8080477.
In situ-forming, biodegradable, and self-healing hydrogels, which maintain their integrity after damage, owing to dynamic interactions, are essential biomaterials for bioapplications, such as tissue engineering and drug delivery. This work aims to develop in situ, biodegradable and self-healable hydrogels based on dynamic covalent bonds between N-succinyl chitosan (S-CHI) and oxidized aldehyde hyaluronic acid (A-HA). A robust effect of the molar ratio of both S-CHI and A-HA was observed on the swelling, mechanical stability, rheological properties and biodegradation kinetics of these hydrogels, being the stoichiometric ratio that which leads to the lowest swelling factor (×12), highest compression modulus (1.1·10−3 MPa), and slowest degradation (9 days). Besides, a rapid (3 s) self-repairing ability was demonstrated in the macro scale as well as by rheology and mechanical tests. Finally, the potential of these biomaterials was evidenced by cytotoxicity essay (>85%).
原位形成、可生物降解且自我修复的水凝胶由于动态相互作用而在受损后仍能保持其完整性，是组织工程和药物递送等生物应用中必不可少的生物材料。这项工作旨在基于N-琥珀酰壳聚糖（S-CHI）和氧化醛透明质酸（A-HA）之间的动态共价键开发原位、可生物降解且可自我修复的水凝胶。观察到S-CHI和A-HA的摩尔比对这些水凝胶的溶胀、机械稳定性、流变学性质和生物降解动力学有显著影响，化学计量比导致最低的溶胀因子（×12）、最高的压缩模量（1.1·10−3 MPa）和最慢的降解（9天）。此外，通过宏观尺度以及流变学和力学测试证明了其快速（3秒）的自我修复能力。最后，细胞毒性试验（>85%）证明了这些生物材料的潜力。
