巴西番樱桃：化学特征分析及细胞毒性潜力评估
Eugenia brasiliensis: Analysis of the Chemical Profile and Evaluation of Cytotoxic Potential.
作者信息
de Oliveira Giovana G F V, Longue Milena F, Pescinelli Letícia M R, Charret Thiago S, Nogueira Thalya S R, Pereira Mariana T M, Vieira Ivo J C, Abreu Lucas S, Pascoal Vinicius D B, Pascoal Aislan C R F
机构信息
Research Laboratory of Natural Products and Bioactive Molecules, Nova Friburgo Health Institute, Fluminense Federal University-UFF, Nova Friburgo, Brazil.
Laboratory of Chemical Sciences, Center for Exact Sciences and Technology, State University of North Fluminense Darcy Ribeiro-UENF, Campos dos Goytacazes, Brazil.
出版信息
Chem Biodivers. 2025 Sep;22(9):e202500429. doi: 10.1002/cbdv.202500429. Epub 2025 May 1.
This work evaluated the antiproliferative potential of Eugenia brasiliensis leaf extracts against the HeLa cervical cancer cell line. The extracts were prepared by maceration using hexane (EBH), dichloromethane (EBD), and ethyl acetate (EBAE), and they were evaluated for their antiproliferative potential through a 3-4,5-dimethyl-thiazol-2-yl-2,5-diphenyltetrazoliumbromide (MTT) assay in the cervical cancer cell culture (HeLa cell line) and a non-cancer cell line (NIH-3T3). EBH, EBD, and EBAE were cytotoxic in HeLa cells, with 50% inhibition concentration (IC) = 97.59, 31.03, and 57.67 µg/mL, respectively. EBD inhibited migration and altered the cell cycle. Eight compounds were tentatively assigned to E. brasiliensis leaf extracts by interpreting their fragmentation patterns and molecular formulae obtained from mass spectra. The dichloromethane extract of the leaves of E. brasiliensis against the cells of cervical cancer showed potential cytotoxicity activity.
本研究评估了巴西番樱桃叶提取物对人宫颈癌HeLa细胞系的抗增殖潜力。通过用己烷（EBH）、二氯甲烷（EBD）和乙酸乙酯（EBAE）浸渍制备提取物，并通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法在宫颈癌细胞培养物（HeLa细胞系）和非癌细胞系（NIH-3T3）中评估它们的抗增殖潜力。EBH、EBD和EBAE对HeLa细胞具有细胞毒性，其50%抑制浓度（IC）分别为97.59、31.03和57.67μg/mL。EBD抑制细胞迁移并改变细胞周期。通过解释从质谱获得的碎片模式和分子式，初步鉴定出8种化合物存在于巴西番樱桃叶提取物中。巴西番樱桃叶的二氯甲烷提取物对宫颈癌细胞显示出潜在的细胞毒性活性。
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