Park Jong-Hun, Park Jeong-Hoon, Lee Sang-Hoon, Yoon Jeong-Jun, Kim Sang-Hyoun, Park Hee-Deung
School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
Clean Innovation Technology Group, Korea Institute of Industrial Technology (KITECH), Jeju-si 63243, Republic of Korea.
Bioresour Technol. 2020 Jun;305:123060. doi: 10.1016/j.biortech.2020.123060. Epub 2020 Feb 20.
In this study, a laboratory-scale acidogenic dynamic membrane bioreactor (DMBR) was operated with or without alginate immobilization under various hydraulic retention times. The interactions between biofilm formation, metabolic flux and predicted functional genes were investigated in the DMBR. Regardless of the alginate immobilization, hydrogen yield was increased after biofilm was formed on a supporting material. Metabolic flux shifts from homoacetogenic to hydrogenic pathways along with biofilm formation was confirmed by flux balance analysis for metabolites. KEGG ortholog count prediction of functional genes from microbial consortia using bioinformatics package (PICRUSt). Predicted enzymes showed similar levels of functional gene expression regardless of the immobilization status. PICRUSt result showed strong evidence of the functional relationship among hydrogen production, biofilm formation, and metabolic pathway. Taken together, this study provides to estimate characteristics of biofilm formation changes and to better understand the microbial metabolism of DMBR.
在本研究中，一个实验室规模的产酸动态膜生物反应器（DMBR）在不同水力停留时间下，分别在有或没有海藻酸钠固定化的情况下运行。研究了DMBR中生物膜形成、代谢通量和预测功能基因之间的相互作用。无论有无海藻酸钠固定化，在支撑材料上形成生物膜后，氢气产量都会增加。通过对代谢物的通量平衡分析证实，随着生物膜的形成，代谢通量从同型产乙酸途径转变为产氢途径。使用生物信息学软件包（PICRUSt）对微生物群落功能基因进行KEGG直系同源计数预测。无论固定化状态如何，预测的酶显示出相似水平的功能基因表达。PICRUSt结果有力地证明了产氢、生物膜形成和代谢途径之间的功能关系。综上所述，本研究有助于估计生物膜形成变化的特征，并更好地理解DMBR的微生物代谢。
