Li Ming, Xu Ting-Min, Zhang Dian-Ying, Zhang Xiao-Meng, Rao Feng, Zhan Si-Zheng, Ma Man, Xiong Chen, Chen Xiao-Feng, Wang Yan-Hua
Department of Trauma and Orthopedics, Peking University People's Hospital; Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education; Trauma Medicine Center, Peking University People's Hospital; National Center for Trauma Medicine, Beijing, China.
Department of Trauma and Orthopedics, Peking University People's Hospital; Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education, Beijing, China.
Neural Regen Res. 2023 Jan;18(1):162-169. doi: 10.4103/1673-5374.344842.
We previously prepared nerve growth factor poly-lactide co-glycolid sustained-release microspheres to treat rat sciatic nerve injury using the small gap sleeve technique. Multiple growth factors play a synergistic role in promoting the repair of peripheral nerve injury; as a result, in this study, we added basic fibroblast growth factors to the microspheres to further promote nerve regeneration. First, in an in vitro biomimetic microenvironment, we developed and used a drug screening biomimetic microfluidic chip to screen the optimal combination of nerve growth factor/basic fibroblast growth factor to promote the regeneration of Schwann cells. We found that 22.56 ng/mL nerve growth factor combined with 4.29 ng/mL basic fibroblast growth factor exhibited optimal effects on the proliferation of primary rat Schwann cells. The successfully prepared nerve growth factor-basic fibroblast growth factor-poly-lactide-co-glycolid sustained-release microspheres were used to treat rat sciatic nerve transection injury using the small gap sleeve bridge technique. Compared with epithelium sutures and small gap sleeve bridging alone, the small gap sleeve bridging technique combined with drug-free sustained-release microspheres has a stronger effect on rat sciatic nerve transfection injury repair at the structural and functional level.
我们之前制备了神经生长因子聚乳酸-乙醇酸共聚物缓释微球，采用小间隙套接技术治疗大鼠坐骨神经损伤。多种生长因子在促进周围神经损伤修复中发挥协同作用；因此，在本研究中，我们在微球中添加碱性成纤维细胞生长因子以进一步促进神经再生。首先，在体外仿生微环境中，我们开发并使用药物筛选仿生微流控芯片筛选神经生长因子/碱性成纤维细胞生长因子的最佳组合，以促进雪旺细胞的再生。我们发现，22.56 ng/mL神经生长因子与4.29 ng/mL碱性成纤维细胞生长因子对原代大鼠雪旺细胞的增殖具有最佳效果。成功制备的神经生长因子-碱性成纤维细胞生长因子-聚乳酸-乙醇酸共聚物缓释微球，采用小间隙套接桥接技术治疗大鼠坐骨神经横断损伤。与单纯上皮缝合和小间隙套接桥接相比，小间隙套接桥接技术联合无药缓释微球对大鼠坐骨神经转染损伤修复在结构和功能水平上具有更强的作用。
