Legüe Marcela, Caneo Mauricio, Aguila Blanca, Pollak Bernardo, Calixto Andrea
Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso 2366103, Chile.
Programa de Doctorado en Microbiología, Universidad de Chile, Santiago de Chile, Chile.
iScience. 2022 Jun 17;25(7):104627. doi: 10.1016/j.isci.2022.104627. eCollection 2022 Jul 15.
The inheritance of memory is an adaptive trait. Microbes challenge the immunity of organisms and trigger behavioral adaptations that can be inherited, but how bacteria produce inheritance of a trait is unknown. We use and its bacteria to study the transgenerational RNA dynamics of interspecies crosstalk leading to a heritable behavior. A heritable response of to microbes is the pathogen-induced diapause (PIDF), a state of suspended animation to evade infection. We identify RsmY, a small RNA involved in quorum sensing in as a trigger of PIDF. The histone methyltransferase (HMT) SET-18/SMYD3 and the argonaute HRDE-1, which promotes multi-generational silencing in the germline, are also needed for PIDF initiation The HMT SET-25/EHMT2 is necessary for memory maintenance in the transgenerational lineage. Our work is a starting point to understanding microbiome-induced inheritance of acquired traits, and the transgenerational influence of microbes in health and disease.
记忆的遗传是一种适应性特征。微生物挑战生物体的免疫力并触发可遗传的行为适应，但细菌如何产生性状的遗传尚不清楚。我们利用秀丽隐杆线虫及其细菌来研究种间串扰导致可遗传行为的跨代RNA动态。秀丽隐杆线虫对微生物的一种可遗传反应是病原体诱导的滞育（PIDF），这是一种为躲避感染而进入的假死状态。我们确定RsmY，一种参与秀丽隐杆线虫群体感应的小RNA，是PIDF的触发因素。组蛋白甲基转移酶（HMT）SET-18/SMYD3和促进种系多代沉默的AGO蛋白HRDE-1，也是启动PIDF所必需的。HMT SET-25/EHMT2对于跨代谱系中的记忆维持是必要的。我们的工作是理解微生物群诱导的获得性性状遗传以及微生物在健康和疾病中的跨代影响的起点。
