Department of Orthopedic Surgery, Guangzhou Hospital of Integrated Traditional and Western Medicine, Guangzhou, PR China.
Pharm Biol. 2022 Dec;60(1):1303-1316. doi: 10.1080/13880209.2022.2093385.
F.C. How. (MO) (Rubiaceae) can strengthen bone function.
To examine the functional mechanism and effect of MO polysaccharides (MOPs) in rats with glucocorticoid-induced osteoporosis (GIOP).
Rats with GIOP were treated with 5, 15 or 45 mL/kg of MOP [ = 15 for each dose, intraperitoneal (i.p.) injection every other day for 8 weeks]. The body weight of rats and histomorphology of bone tissues were examined. Bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (Exo) were collected and identified. Bone marrow-derived macrophages (BMMs) were induced to differentiate into osteoclasts and treated with BMSC-Exo for studies.
MOP reduced the body weight (5, 15, or 45 mg/kg MOP vs. phosphate-buffered saline: 8%, 15% and 25%, < 0.01), elevated the bone volume to tissue volume (BV/TV), mean trabecular thickness (Tb.Th), mean trabecular number (Tb.N) and mean connectivity density (Conn.D) (40-86%, < 0.01), decreased the mean trabecular separation/spacing (Tb.Sp) (22-37%, < 0.01), increased the cortical bone continuity (35-90%, < 0.01) and elevated RUNX family transcription factor 2 and RANK levels (5-12%, < 0.01), but suppressed matrix metallopeptidase 9 and cathepsin K levels (9-20%, < 0.01) in femur tissues. BMSC-Exo from MOP-treated rats (MOP-Exo) suppressed osteoclastic differentiation and proliferation of BMMs. The downregulation of microRNA-101-3p (miR-101-3p) or the upregulation of prostaglandin-endoperoxide synthase 2 (PTGS2) blocked the functions of MOP-Exo.
MOP inhibits osteoclastic differentiation and could potentially be used for osteoporosis management. This suppression may be enhanced by the upregulation of miR-101-3p or the inhibition of PTGS2.
五指牛奶（MO）（茜草科）可增强骨骼功能。
研究 MO 多糖（MOPs）在糖皮质激素诱导骨质疏松症（GIOP）大鼠中的作用机制和效果。
采用腹腔注射 5、15 或 45ml/kg MOP[剂量分别为 15 只，每 2 天 1 次，共 8 周]的方法建立 GIOP 大鼠模型。检测大鼠体重和骨组织形态学变化。收集骨髓间充质干细胞（BMSC）来源的外泌体（Exo）并进行鉴定。诱导骨髓来源的巨噬细胞（BMM）分化为破骨细胞，用 BMSC-Exo 处理进行研究。
MOP 降低了体重（5、15 或 45mg/kg MOP 与磷酸盐缓冲液相比：8%、15%和 25%，<0.01），增加了骨体积/组织体积（BV/TV）、平均骨小梁厚度（Tb.Th）、平均骨小梁数量（Tb.N）和平均连接密度（Conn.D）（40-86%，<0.01），降低了平均骨小梁间距（Tb.Sp）（22-37%，<0.01），增加了皮质骨连续性（35-90%，<0.01），并提高了 RUNX 家族转录因子 2 和 RANK 水平（5-12%，<0.01），但降低了股骨组织中基质金属蛋白酶 9 和组织蛋白酶 K 水平（9-20%，<0.01）。来自 MOP 处理大鼠的 BMSC-Exo（MOP-Exo）抑制了破骨细胞的分化和 BMM 的增殖。下调 microRNA-101-3p（miR-101-3p）或上调前列腺素内过氧化物合酶 2（PTGS2）可阻断 MOP-Exo 的功能。
MOP 抑制破骨细胞分化，可能用于骨质疏松症的治疗。这种抑制作用可能通过 miR-101-3p 的上调或 PTGS2 的抑制来增强。
