长链非编码 RNA MEG3 通过靶向 miR-145-5p/KLF4 诱导小鼠来源的脂肪干细胞的内皮分化。

LncRNA MEG3 induces endothelial differentiation of mouse derived adipose-derived stem cells by targeting MiR-145-5p/KLF4.

机构信息

Department of Dermatology, First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People's Republic of China.

Department of Medicine, Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People's Republic of China.

出版信息

Mol Biol Rep. 2022 Sep;49(9):8495-8505. doi: 10.1007/s11033-022-07671-z. Epub 2022 Jul 8.

DOI:10.1007/s11033-022-07671-z

PMID:35802277

Abstract

BACKGROUND

The present study aimed to investigate the mechanisms through which long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) affected the endothelial differentiation of mouse derived adipose-derived stem cells (ADSCs).

MATERIALS AND METHODS

ADSCs were isolated and identified by specific surface marker detection. The effects of lncRNA MEG3 on endothelial differentiation of ADSCs were also detected via quantitative PCR, western blotting, immunofluorescence and Matrigel angiogenesis assays. In addition, using target gene prediction tools and luciferase reporter assays, the downstream target gene was demonstrated.

RESULTS

LncRNA MEG3 targeted and reduced the expression levels of microRNA-145-5p (miR-145-5p), which upregulated the expression levels of Krüppel like factor 4 (KLF4), promoting endothelial differentiation of ADSCs.

CONCLUSION

LncRNA MEG3 induced endothelial differentiation of ADSCs by targeting miR-145-5p/KLF4, which may provide novel insights to illustrate the mechanism of endothelial differentiation of ADSCs.

摘要