Department of Dermatology, First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People's Republic of China.
Department of Medicine, Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People's Republic of China.
Mol Biol Rep. 2022 Sep;49(9):8495-8505. doi: 10.1007/s11033-022-07671-z. Epub 2022 Jul 8.
The present study aimed to investigate the mechanisms through which long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) affected the endothelial differentiation of mouse derived adipose-derived stem cells (ADSCs).
ADSCs were isolated and identified by specific surface marker detection. The effects of lncRNA MEG3 on endothelial differentiation of ADSCs were also detected via quantitative PCR, western blotting, immunofluorescence and Matrigel angiogenesis assays. In addition, using target gene prediction tools and luciferase reporter assays, the downstream target gene was demonstrated.
LncRNA MEG3 targeted and reduced the expression levels of microRNA-145-5p (miR-145-5p), which upregulated the expression levels of Krüppel like factor 4 (KLF4), promoting endothelial differentiation of ADSCs.
LncRNA MEG3 induced endothelial differentiation of ADSCs by targeting miR-145-5p/KLF4, which may provide novel insights to illustrate the mechanism of endothelial differentiation of ADSCs.
本研究旨在探讨长链非编码 RNA（lncRNA）母系表达基因 3（MEG3）影响小鼠来源脂肪干细胞（ADSCs）内皮分化的机制。
通过特异性表面标志物检测分离和鉴定 ADSCs。通过定量 PCR、western blot、免疫荧光和 Matrigel 血管生成实验检测 lncRNA MEG3 对 ADSCs 内皮分化的影响。此外，利用靶基因预测工具和荧光素酶报告基因实验，证明下游靶基因。
lncRNA MEG3 靶向并降低了 microRNA-145-5p（miR-145-5p）的表达水平，上调了 Krüppel 样因子 4（KLF4）的表达水平，促进了 ADSCs 的内皮分化。
lncRNA MEG3 通过靶向 miR-145-5p/KLF4 诱导 ADSCs 的内皮分化，这可能为阐明 ADSCs 内皮分化的机制提供新的见解。
