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长链非编码 RNA LINC00314 通过 hsa-miR-129-5p/GRM5 轴通过 Wnt 信号通路促进脂肪来源干细胞的成骨分化。

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway.

机构信息

Department of Orthopedics, The Second Hospital of Hebei Medical University, No. 215, Hepingxi Road, Shijiazhuang, 050000, Hebei Province, China.

出版信息

Stem Cell Res Ther. 2020 Jun 17;11(1):240. doi: 10.1186/s13287-020-01754-z.

DOI:10.1186/s13287-020-01754-z
PMID:32552820
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7302136/
Abstract

BACKGROUND

Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs.

METHODS

LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs.

RESULTS

LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway.

CONCLUSIONS

LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.

摘要

背景

许多研究表明，长非编码 RNA（lncRNA）与脂肪来源干细胞（ADSCs）成骨分化的刺激和骨质疏松的预防密切相关。目前的研究旨在探讨新的 lncRNA，并探讨 LINC00314/miR-129-5p/GRM5 轴在调节 ADSC 成骨分化中的作用和分子机制。

方法

对正常和成骨诱导的 ADSC（成骨组）进行 lncRNA 和 miRNA 测序。通过 R 软件获得异常表达的 lncRNA 和 miRNA，通过 RT-PCR 测量 LINC00314、miR-129-5p 和 GRM5 在成骨诱导过程中的相对表达。然后将 pcDNA3.1-sh-LINC00314 和 agomiR-129-5p 转染入 ADSC。进行茜素红染色（ARS）和碱性磷酸酶（ALP）染色，以鉴定 LINC00314/miR-129-5p/GRM5 轴在调节 ADSC 成骨分化中的作用机制。

结果

LINC00314 在成骨诱导的 ADSC 中显著上调。LINC00314 和 GRM5 模拟物增加了成骨分化的早期和晚期标志物，不仅 ALP 活性显著增加，而且钙沉积也更高，而 miR-129-5p 模拟物则有相反的作用。通过荧光素酶报告基因检测证实 LINC00314 直接靶向 miR-129-5p，miR-129-5p 抑制 GRM5 表达。此外，LINC00314/miR-129-5p/GRM5 调节轴激活了 Wnt/β-catenin 信号通路。

结论

LINC00314 在 ADSC 的成骨分化中发挥促进作用，因此 LINC00314/miR-129-5p/GRM5 轴可能是成骨相关疾病的一种新机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/9a781aeaf6ca/13287_2020_1754_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/bbf92a3b0a54/13287_2020_1754_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/010236d30701/13287_2020_1754_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/476e379b583e/13287_2020_1754_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/9a781aeaf6ca/13287_2020_1754_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/bbf92a3b0a54/13287_2020_1754_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/010236d30701/13287_2020_1754_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/476e379b583e/13287_2020_1754_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0061/7302136/9a781aeaf6ca/13287_2020_1754_Fig5_HTML.jpg

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2
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Pathol Res Pract. 2019 Dec;215(12):152641. doi: 10.1016/j.prp.2019.152641. Epub 2019 Oct 4.
3
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BDJ Open. 2024 Jun 5;10(1):45. doi: 10.1038/s41405-024-00232-5.
4
Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives.基于脂肪干细胞的肌肉骨骼再生优化策略：最新进展与展望。
Stem Cell Res Ther. 2024 Mar 27;15(1):91. doi: 10.1186/s13287-024-03703-6.
5
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RNA Biol. 2024 Jan;21(1):1-13. doi: 10.1080/15476286.2023.2290771. Epub 2023 Dec 22.
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8
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5
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6
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