• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-487b 通过靶向抑制 Ifitm3 抑制脊髓损伤中的炎症和神经元凋亡。

MiR-487b suppressed inflammation and neuronal apoptosis in spinal cord injury by targeted Ifitm3.

机构信息

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China.

Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China.

出版信息

Metab Brain Dis. 2022 Oct;37(7):2405-2415. doi: 10.1007/s11011-022-01015-3. Epub 2022 Jul 8.

DOI:10.1007/s11011-022-01015-3
PMID:35802304
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9581865/
Abstract

Spinal cord injury (SCI) was a serious nerve injury, which involves complex genetic changes. This paper was intended to investigate the function and mechanism of differentially expressed genes in SCI. The three datasets GSE92657, GSE93561 and GSE189070 of SCI from GEO database were used to identify differentially expressed genes (DEGs). We identified the common DEGs in the three datasets GSE92657, GSE93561 and GSE189070 of SCI from GEO database. Next, a protein-protein interaction (PPI) network of DEGs was constructed. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were significantly enriched in immune response, inflammatory response. The expression level of immune-related genes (Arg1, Ccl12, Ccl2, Ifitm2, Ifitm3, and et al.) at different time points of SCI were analyzed in GSE189070 dataset. Next, differentially expressed miRNAs (DE-miRNAs) were identified in SCI compared with normal based on GSE158194 database. DE-miRNA and targeted immune-related genes were predicted by miRwalk, including miR-487b-5p targeted Ifitm3, miR-3072-5p targeted Ccl3, and et al. What's more, the miR-487b was identified and verified to be down-regulated in Lipopolysaccharide (LPS)-induced BV-2 cell model. Further, the miR-487b inhibited cell inflammation and apoptosis in LPS-induced BV2 cell by targeted Ifitm3. For the first time, our results revealed that miR-487b may play an important regulatory role in SCI by targeted Ifitm3 and provide further evidence for SCI research.

摘要

脊髓损伤 (SCI) 是一种严重的神经损伤，涉及复杂的遗传变化。本文旨在研究 SCI 差异表达基因的功能和机制。从 GEO 数据库中使用三个 SCI 数据集 GSE92657、GSE93561 和 GSE189070 来识别差异表达基因 (DEGs)。我们鉴定了 GSE92657、GSE93561 和 GSE189070 三个数据集的 SCI 中的共同 DEGs。接下来，构建了一个 DEG 的蛋白质-蛋白质相互作用 (PPI) 网络。随后，基因本体论 (GO) 和京都基因与基因组百科全书 (KEGG) 分析表明，DEGs 在免疫反应、炎症反应中显著富集。在 GSE189070 数据集分析了 SCI 不同时间点免疫相关基因（Arg1、Ccl12、Ccl2、Ifitm2、Ifitm3 等）的表达水平。接下来，基于 GSE158194 数据库，比较 SCI 与正常对照之间的差异表达 miRNA (DE-miRNA)。通过 miRwalk 预测 DE-miRNA 和靶向免疫相关基因，包括 miR-487b-5p 靶向 Ifitm3、miR-3072-5p 靶向 Ccl3 等。此外，还鉴定并验证了 miR-487b 在脂多糖 (LPS) 诱导的 BV-2 细胞模型中下调。进一步，miR-487b 通过靶向 Ifitm3 抑制 LPS 诱导的 BV2 细胞炎症和凋亡。首次表明，miR-487b 可能通过靶向 Ifitm3 在 SCI 中发挥重要调节作用，为 SCI 研究提供了进一步证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/553a849b7b0b/11011_2022_1015_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/8b0b23a87867/11011_2022_1015_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/c21f4f983654/11011_2022_1015_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/8ad40d1286f1/11011_2022_1015_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/173ef9d0c393/11011_2022_1015_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/553a849b7b0b/11011_2022_1015_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/8b0b23a87867/11011_2022_1015_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/c21f4f983654/11011_2022_1015_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/8ad40d1286f1/11011_2022_1015_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/173ef9d0c393/11011_2022_1015_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/026d/9581865/553a849b7b0b/11011_2022_1015_Fig5_HTML.jpg

相似文献

1
MiR-487b suppressed inflammation and neuronal apoptosis in spinal cord injury by targeted Ifitm3.miR-487b 通过靶向抑制 Ifitm3 抑制脊髓损伤中的炎症和神经元凋亡。
Metab Brain Dis. 2022 Oct;37(7):2405-2415. doi: 10.1007/s11011-022-01015-3. Epub 2022 Jul 8.
2
Integrated analysis of competing endogenous RNA (ceRNA) networks in subacute stage of spinal cord injury.脊髓损伤亚急性期竞争性内源性 RNA（ceRNA）网络的综合分析。
Gene. 2020 Feb 5;726:144171. doi: 10.1016/j.gene.2019.144171. Epub 2019 Oct 26.
3
Regulation of gene expression in rats with spinal cord injury based on microarray data.基于微阵列数据的大鼠脊髓损伤基因表达调控
Mol Med Rep. 2015 Aug;12(2):2465-72. doi: 10.3892/mmr.2015.3670. Epub 2015 Apr 23.
4
MicroRNA-129-5p alleviates spinal cord injury in mice via suppressing the apoptosis and inflammatory response through HMGB1/TLR4/NF-κB pathway.微小 RNA-129-5p 通过抑制 HMGB1/TLR4/NF-κB 通路的细胞凋亡和炎症反应缓解小鼠脊髓损伤。
Biosci Rep. 2020 Mar 27;40(3). doi: 10.1042/BSR20193315.
5
Key Genes Associated with Pyroptosis in Gout and Construction of a miRNA-mRNA Regulatory Network.与痛风中细胞焦亡相关的关键基因及 miRNA-mRNA 调控网络的构建。
Cells. 2022 Oct 17;11(20):3269. doi: 10.3390/cells11203269.
6
Identification of a circRNA-mediated comprehensive ceRNA network in spinal cord injury pathogenesis.鉴定在脊髓损伤发病机制中的环状 RNA 介导的全面 ceRNA 网络。
Exp Biol Med (Maywood). 2022 Jun;247(11):931-944. doi: 10.1177/15353702221082929. Epub 2022 Apr 11.
7
Biomarkers mining for spinal cord injury based on integrated multi-transcriptome expression profile data.基于整合多转录组表达谱数据的脊髓损伤生物标志物挖掘。
J Orthop Surg Res. 2021 Apr 16;16(1):267. doi: 10.1186/s13018-021-02392-8.
8
Identification and bioinformatics analysis of genes associated with pyroptosis in spinal cord injury of rat and mouse.鉴定和生物信息学分析与大鼠和小鼠脊髓损伤相关的细胞焦亡基因。
Sci Rep. 2024 Jun 18;14(1):14023. doi: 10.1038/s41598-024-64843-6.
9
Uncovering the shared neuro-immune-related regulatory mechanisms between spinal cord injury and osteoarthritis.揭示脊髓损伤与骨关节炎之间共享的神经免疫相关调节机制。
Heliyon. 2024 Apr 25;10(9):e30336. doi: 10.1016/j.heliyon.2024.e30336. eCollection 2024 May 15.
10
Identification of key autophagy-related genes and pathways in spinal cord injury.鉴定脊髓损伤中的关键自噬相关基因和途径。
Sci Rep. 2024 Mar 19;14(1):6553. doi: 10.1038/s41598-024-56683-1.

引用本文的文献

1
Immune characteristics of olfactory ensheathing cells and repair of nerve injury.嗅鞘细胞的免疫特性与神经损伤修复
Front Immunol. 2025 May 15;16:1571573. doi: 10.3389/fimmu.2025.1571573. eCollection 2025.
2
Novel link between plasma bilirubin and anti-inflammatory miRNA profiles in follicular fluid of IVF patients.体外受精患者卵泡液中血浆胆红素与抗炎性微小RNA谱之间的新联系。
Am J Physiol Endocrinol Metab. 2025 Jun 1;328(6):E1001-E1012. doi: 10.1152/ajpendo.00479.2024. Epub 2025 May 5.
3
Plasma miRNA Biomarker Signatures in Parkinsonian Syndromes.

本文引用的文献

1
MiRNA-411 attenuates inflammatory damage and apoptosis following spinal cord injury.miRNA-411 减轻脊髓损伤后的炎症损伤和细胞凋亡。
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):491-498. doi: 10.26355/eurrev_202001_20022.
帕金森综合征中的血浆微小RNA生物标志物特征
Mol Neurobiol. 2025 Apr 4. doi: 10.1007/s12035-025-04890-w.
4
MiRNA-223-5p inhibits hypoxia-induced apoptosis of BMSCs and promotes repair in Legg-Calvé-Perthes disease by targeting CHAC2 and activating the Wnt/β-catenin signaling pathway.微小RNA-223-5p通过靶向CHAC2并激活Wnt/β-连环蛋白信号通路，抑制缺氧诱导的骨髓间充质干细胞凋亡，促进Legg-Calvé-Perthes病的修复。
PLoS One. 2025 Jan 24;20(1):e0315230. doi: 10.1371/journal.pone.0315230. eCollection 2025.
5
Resveratrol Upregulates miR-124-3p Expression to Target DAPK1, Regulating the NLRP3/Caspase-1/GSDMD Pathway to Inhibit Pyroptosis and Alleviate Spinal Cord Injury.白藜芦醇上调miR-124-3p表达以靶向DAPK1，调节NLRP3/半胱天冬酶-1/GSDMD途径以抑制细胞焦亡并减轻脊髓损伤。
J Cell Mol Med. 2025 Jan;29(2):e70338. doi: 10.1111/jcmm.70338.
6
Exosomal miR-17-92 Cluster from BMSCs Alleviates Apoptosis and Inflammation in Spinal Cord Injury.骨髓间充质干细胞来源的外泌体miR-17-92簇减轻脊髓损伤中的细胞凋亡和炎症
Biochem Genet. 2024 Jul 3. doi: 10.1007/s10528-024-10876-5.
7
Research hotspots and trends of microRNAs in spinal cord injury: a comprehensive bibliometric analysis.脊髓损伤中微小RNA的研究热点与趋势：一项全面的文献计量分析
Front Neurol. 2024 May 21;15:1406977. doi: 10.3389/fneur.2024.1406977. eCollection 2024.
8
Circ-KATNAL1 Knockdown Reduces Neuronal Apoptosis and Alleviates Spinal Cord Injury Through the miR-98-5p/PRDM5 Regulatory Axis.环状 RNA-KATNAL1 敲低通过 miR-98-5p/PRDM5 调控轴减少神经元凋亡并缓解脊髓损伤。
Mol Biotechnol. 2024 Oct;66(10):2841-2849. doi: 10.1007/s12033-023-00895-9. Epub 2023 Sep 27.
9
Interferon-induced transmembrane protein 3 in the hippocampus: a potential novel target for the therapeutic effects of recombinant human brain natriuretic peptide on sepsis-associated encephalopathy.海马体中的干扰素诱导跨膜蛋白3：重组人脑利钠肽对脓毒症相关性脑病治疗作用的潜在新靶点。
Front Mol Neurosci. 2023 Aug 1;16:1182005. doi: 10.3389/fnmol.2023.1182005. eCollection 2023.
10
lncRNA XIST inhibition promotes M2 polarization of microglial and aggravates the spinal cord injury via regulating miR-124-3p / IRF1 axis.长链非编码RNA XIST抑制通过调节miR-124-3p/IRF1轴促进小胶质细胞的M2极化并加重脊髓损伤。
Heliyon. 2023 Jul 3;9(7):e17852. doi: 10.1016/j.heliyon.2023.e17852. eCollection 2023 Jul.