Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq; Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Kurdistan Region, Iraq.
Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq.
Pathol Res Pract. 2022 Aug;236:154009. doi: 10.1016/j.prp.2022.154009. Epub 2022 Jul 1.
Cyclin D1 has been shown to participate in the pathogenesis of breast cancer. This cell cycle-related protein has direct or indirect interactions with long non-coding RNAs (lncRNAs). In the present two-step study, we first identified CCND1-related lncRNAs through an in silico approach. Then, we measured expression of CCND1 mRNA and five lncRNAs in paired breast cancer samples and their matched non-cancerous samples obtained from adjacent tissues. HOTTIP expression was significantly higher in breast cancer tissues compared with adjacent tissues (expression ratio (95% CI)= 4.63 (1.56-13.76), P value= 0.0070). Similarly, CBR3-AS1 was up-regulated in cancerous tissues compared with control tissues (expression ration (95% CI)= 3.26 (1.35-7.86), P value= 0.0122). Expression of HOTTIP was higher in estrogen receptor (ER) negative samples compared with ER positive ones (-4.35 ± 1.33 versus -4.63 ± 0.62, P value=0.002). CBR3-AS1 could differentiate between these two sets of samples with AUC±SD, sensitivity, specificity and P values of 0.7 ± 0.05, 0.9, 0.49 and 0.003, respectively. These values were 0.68 ± 0.04, 0.87, 0.34 and 0.04 for HOTTIP. Although we could not find difference in expression of CCND1 between these two sets of samples, we reported up-regulation of two CCND1-related lncRNAs in breast cancer samples. These lncRNAs are putative markers for breast cancer.
Cyclin D1 已被证明参与乳腺癌的发病机制。这种细胞周期相关蛋白与长链非编码 RNA（lncRNA）直接或间接相互作用。在本研究的两步法中，我们首先通过计算方法鉴定了与 CCND1 相关的 lncRNA。然后，我们测量了配对的乳腺癌样本及其来自相邻组织的匹配非癌样本中 CCND1 mRNA 和五种 lncRNA 的表达。HOTTIP 在乳腺癌组织中的表达明显高于相邻组织（表达比（95%CI）=4.63（1.56-13.76），P 值=0.0070）。同样，CBR3-AS1 在癌组织中的表达高于对照组织（表达比（95%CI）=3.26（1.35-7.86），P 值=0.0122）。在雌激素受体（ER）阴性样本中，HOTTIP 的表达高于 ER 阳性样本（-4.35±1.33 与-4.63±0.62，P 值=0.002）。CBR3-AS1 可以用 AUC±SD、灵敏度、特异性和 P 值来区分这两组样本，分别为 0.7±0.05、0.9、0.49 和 0.003。HOTTIP 的这些值分别为 0.68±0.04、0.87、0.34 和 0.04。虽然我们没有发现这两组样本中 CCND1 的表达存在差异，但我们报道了在乳腺癌样本中两个 CCND1 相关 lncRNA 的上调。这些 lncRNA 是乳腺癌的潜在标志物。
