Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furou-chou, Chikusa, Nagoya, Aichi, Japan.
Biosci Biotechnol Biochem. 2022 Aug 24;86(9):1183-1191. doi: 10.1093/bbb/zbac113.
The YggS/PLPBP protein (also called COG0325 or PLPHP) is a conserved pyridoxal 5'-phosphate (PLP)-binding protein present in all 3 domains of life. Recent studies have demonstrated that disruption or mutation of this protein has multifaceted effects in various organisms, including vitamin B6-dependent epilepsy in humans. In Escherichia coli, disruption of this protein-encoded by yggS-perturbs Thr-Ile/Val metabolism, one-carbon metabolism, coenzyme A synthesis, and vitamin B6 homeostasis. This protein is critical for maintaining low levels of pyridoxine 5'-phosphate (PNP) in various organisms. In the yggS-deficient E. coli strain, inhibition of PLP-dependent enzymes, such as the glycine cleavage system by PNP, is the root cause of metabolic perturbation. Our data suggest that the YggS/PLPBP protein may be involved in the balancing of B6 vitamers by mediating efficient turnover of protein-bound B6 vitamers. This paper reviews recent findings on the function of the YggS/PLPBP protein.
YggS/PLPBP 蛋白（也称为 COG0325 或 PLPHP）是一种保守的吡哆醛 5′-磷酸（PLP）结合蛋白，存在于所有三个生命领域。最近的研究表明，该蛋白的破坏或突变在包括人类维生素 B6 依赖性癫痫在内的各种生物体中具有多方面的影响。在大肠杆菌中，该蛋白（由 yggS 编码）的破坏扰乱了苏氨酸-异亮氨酸/缬氨酸代谢、一碳代谢、辅酶 A 合成和维生素 B6 稳态。该蛋白对于维持各种生物体中低水平的吡哆醇 5′-磷酸（PNP）至关重要。在 yggS 缺陷型大肠杆菌菌株中，PNP 抑制 PLP 依赖性酶，如甘氨酸裂解系统，是代谢紊乱的根本原因。我们的数据表明，YggS/PLPBP 蛋白可能通过介导蛋白结合的 B6 类维生素的有效周转，参与 B6 类维生素的平衡。本文综述了 YggS/PLPBP 蛋白功能的最新发现。
